Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture media, Other biological fluids, Plasma, Serum, Urine
- Sensitivity: 10 µM
製品名Ammonia Assay Kit - Modified Berthelot - (Colorimetric)
Ammonia キット 製品一覧
サンプルの種類Urine, Serum, Plasma, Other biological fluids, Cell culture media
検出感度< 10 µM
Ammonia Assay Kit - Modified Berthelot (Colorimetric) ab102509 provides a rapid, simple, sensitive, and reliable assay to measure ammonia.
In this non-enzymatic ammonia assay protocol, ammonia is used to form indophenol, a highly colored product easily quantifiable by colorimetry (λ=670nm) using a plate reader.
The kit can detect less than 1 nmol(~10 µM) ammonia or ammonium using either a 96 well or 384 well microwell plate.
Ammonia assay protocol summary:
- add samples and standards to wells
- add reagent 1 and reagent 2 and incubate for 30 min
- analyze with a microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K470 Ammonia Colorimetric Assay Kit II. K470-100 is the same size as the 100 test size of ab102509.
Ammonia assay kit ab83360 is an alternative to this product, and is the most popular ammonia assay kit.
保存方法Store at +4°C. Please refer to protocols.
内容 ラベル 100 tests Ammonia Reagent 1 Amber 1 x 8ml Ammonia Reagent 2 Clear 1 x 4ml Ammonium Chloride Standard Yellow 1 x 0.1ml
関連性Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology like normal animal acid/base balance.
ab102509 は 2 報の論文で使用されています。
- Raghavan SS et al. Development and application of a transcriptional sensor for detection of heterologous acrylic acid production in E. coli. Microb Cell Fact 18:139 (2019). PubMed: 31426802
- Wu C et al. Fluxomic Analysis Reveals Central Carbon Metabolism Adaptation for Diazotroph Azotobacter vinelandii Ammonium Excretion. Sci Rep 9:13209 (2019). PubMed: 31520074