Anti-Amino-terminal enhancer of split 抗体 (ab16638)


  • 製品名
    Anti-Amino-terminal enhancer of split antibody
    Amino-terminal enhancer of split 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Amino-terminal enhancer of split
  • 由来種
  • アプリケーション
    適用あり: IP, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human GRG (Groucho homologue).

    (Peptide available as ab16684.)

  • ポジティブ・コントロール
    • This antibody gives a positive signal in WB against Recombinant His-tagged GRG



Our Abpromise guarantee covers the use of ab16638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 22 kDa).


  • 機能
    Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.
  • 組織特異性
    Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
  • 配列類似性
    Belongs to the WD repeat Groucho/TLE family.
  • ドメイン
    Lacks the C-terminal WD repeats.
  • 翻訳後修飾
    Ubiquitinated by XIAP/BIRC4.
  • 細胞内局在
  • Information by UniProt
  • 参照データベース
  • 別名
    • Aes 1 antibody
    • Aes 2 antibody
    • aes antibody
    • AES_HUMAN antibody
    • Aes1 antibody
    • Aes2 antibody
    • Amino enhancer of split antibody
    • Amino terminal enhancer of split antibody
    • Amino-terminal enhancer of split antibody
    • Esp 1 antibody
    • Esp1 antibody
    • Gp130 associated protein GAM antibody
    • Gp130-associated protein GAM antibody
    • GRG 5 antibody
    • GRG antibody
    • GRG protein antibody
    • Grg-5 antibody
    • GRG5 antibody
    • Groucho-related protein 5 antibody
    • Protein ESP 1 antibody
    • Protein ESP1 antibody
    • Protein GRG antibody
    • TLE 5 antibody
    • TLE5 antibody
    see all


  • All lanes : Anti-Amino-terminal enhancer of split antibody (ab16638) at 1 µg/ml

    Lanes 6-10 : Human Amino-terminal enhancer of split peptide (ab16684) at 1 µg/ml

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 21 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 26 kDa (possible cross reactivity), 28 kDa (possible cross reactivity), 33 kDa (possible cross reactivity), 40 kDa (possible cross reactivity), 53 kDa (possible cross reactivity), 75 kDa (possible cross reactivity), 80 kDa (possible cross reactivity)

    1 - 10 : GRG (Groucho homologue) antibody (ab16638) at 1 ug/ml
    6 - 10 : GRG (Groucho homologue) peptide (ab16684) at 1 ug/ml
    1, 6: Recombinant His-tagged GRG (28ug)
    2, 7: Human muscle (20ug)
    3, 8: Human heart (20ug)
    4, 9: Human embryonic stem cell lysate
    5, 10: Mouse embryonic stem cell lysate

    Observed band size : 32 kDa (arrow)
    Additional bands at : 21 kDa (possible non-specific binding, possible endogenous GRG), 28 kDa (possible non-specific binding, possible endogenous GRG), 35 kDa (possible non-specific binding), 40 kDa (possible non-specific binding), 53 kDa (possible non-specific binding), 75 kDa (possible non-specific binding), 80 kDa (possible non-specific binding).

    A major band coresponding to GRG was observed in HEK293 lysates in which His-tagged GR had been overexpressed. Many


ab16638 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you very much for your interest in ab86016, ab16638 and ab62555. To our knowledge, these antibodies have not been tested in with IF. ab16638, although predicted to react with mouse GRG, this has also not been tested as yet. Therefore, I c...

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Thank you for your patience. I have received confirmation that the sequence from this antibody cross reacts with Q08117 (197AA long) and Q14CJ1 (264AA long). There is no indication of this cross reacting with any other forms though on looking at Q08...

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