リコンビナントRabMAb

Anti-AMH 抗体 [EPR23380-171] - BSA and Azide free (ab272227)

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Immunohistochemistry (Frozen sections) - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (ab272227)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (ab272227)
  • Immunohistochemistry (Frozen sections) - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (ab272227)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (ab272227)
  • Anti-AMH antibody [EPR23380-171] - BSA and Azide free (ab272227)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23380-171] to AMH - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

製品の概要

  • 製品名

    Anti-AMH antibody [EPR23380-171] - BSA and Azide free
    AMH 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23380-171] to AMH - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Mouse ovary tissue; Rat ovary tissue. IHC-Fr: Mouse ovary tissue; Rat ovary tissue.

  • 特記事項

    ab272227 is the carrier-free version of ab272221. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-Fr
Mouse
IHC-P
Mouse
アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
  • 追加情報
    Is unsuitable for IP or WB.

    • ターゲット情報

    • 関連性

      Anti mullerian hormone (AMH) is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulphide linked precursor that is cleaved to release the mature 30kD homodimer. Originally classified as a foetal testicular hormone that inhibits Mullerian duct development, AMH is expressed post natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth.

    • 細胞内局在

      Secreted

    • 参照データベース

      • 別名

        • Anti muellerian hormone antibody
        • MIF antibody
        • MIS antibody
        • Muellerian inhibiting factor antibody
        • Mullerian inhibiting substance antibody
        see all

      画像

      • Immunohistochemistry (Frozen sections) - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (ab272227)
        Immunohistochemistry (Frozen sections) - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (ab272227)

        4% PFA-fixed, 0.2% Triton X-100 permeabilised rat ovary tissue labeling AMH with ab272221 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Cytoplasmic staining on granulosa cells of follicle.The nuclear counter stain is DAPI (Blue).

        Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

        Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272221).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (ab272227)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (ab272227)

        Immunohistochemical analysis of paraffin-embedded Mouse ovary tissue labeling AMH with ab272221 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulosa cells of mouse ovary. The section was incubated with ab272221 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272221).

      • Immunohistochemistry (Frozen sections) - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (ab272227)
        Immunohistochemistry (Frozen sections) - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (ab272227)

        4% PFA-fixed, 0.2% Triton X-100 permeabilised mouse ovary tissue labeling AMH with ab272221 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Cytoplasmic staining on granulosa cells of follicle.The nuclear counter stain is DAPI (Blue).

        Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

        Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272221).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (ab272227)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR23380-171] - BSA and Azide free (ab272227)

        Immunohistochemical analysis of paraffin-embedded Rat ovary tissue labeling AMH with ab272221 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulosa cells of rat ovary. The section was incubated with ab272221 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272221).

      • Anti-AMH antibody [EPR23380-171] - BSA and Azide free (ab272227)
        Anti-AMH antibody [EPR23380-171] - BSA and Azide free (ab272227)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab272227 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab272227 は論文での使用が確認できていません。

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