Application
IHC - Wholemount
Sample
Mouse Embryo (E12.5 Mouse Embryo)
Specification
E12.5 Mouse Embryo
Other product details
Incubation time
99 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: PBS 1x +0.5% Gelatin +0.5% Triton
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Abcam antibody:Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed
Additional data
Additional Notes
4%PFA immersion-fixed (1 day)
Incubated for 12 days at 37°C in primary antibody
then cleared using iDisco+ protocol
Imaged with light sheet microscopy
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Alain Chedotal
Verified customer
投稿 Jan 31 2022