Anti-ALPPL2 抗体 [EPR20905-21] - BSA and Azide free (ab269957)
![Western blot - Anti-ALPPL2 antibody [EPR20905-21] - BSA and Azide free (ab269957)](https://www.abcam.co.jp/ps/products/269/ab269957/Images/ab269957-391012-antialppl2antibodyepr2090521westernblotnes.jpg "Western blot - Anti-ALPPL2 antibody [EPR20905-21] - BSA and Azide free (ab269957)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20905-21] to ALPPL2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
製品の概要
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製品名
Anti-ALPPL2 antibody [EPR20905-21] - BSA and Azide free
ALPPL2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR20905-21] to ALPPL2 - BSA and Azide free -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: PANC-1, Capan-1 and nES whole cell lysates.
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特記事項
ab269957 is the carrier-free version of ab235581. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR20905-21 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| WB |
Human
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| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
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| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa). |
ターゲット情報
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組織特異性
Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors. -
配列類似性
Belongs to the alkaline phosphatase family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 251 Human
- Omim: 171810 Human
- SwissProt: P10696 Human
- Unigene: 333509 Human
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別名
- Akp5 antibody
- Alkaline phosphatase antibody
- Alkaline phosphatase Nagao isozyme antibody
see all
画像
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Western blot - Anti-ALPPL2 antibody [EPR20905-21] - BSA and Azide free (ab269957)All lanes : Anti-ALPPL2 antibody [EPR20905-21] (ab235581) at 1/1000 dilution
Lane 1 : nES (human native embryonic stem cell), whole cell lysate
Lane 2 : pES (human primed embryonic stem cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 57 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysates were kindly provided by Dr Shaorong Gao.
140KD band is dimer (PMID: 23467613).
Exposure time: 5.5 seconds.
These data were developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab235581).
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![Anti-ALPPL2 antibody [EPR20905-21] - BSA and Azide free (ab269957)](https://www.abcam.co.jp/ps/products/269/ab269957/Images/ab269957-2-benefits-of-recombinant-antibodies.png)
Anti-ALPPL2 antibody [EPR20905-21] - BSA and Azide free (ab269957)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab269957 は論文での使用が確認できていません。