Western blot abreview for Anti-alpha Tubulin antibody [TU-01]

Western blot

Plants Tissue lysate - whole (Nicotiana benthamiana)

Non-reduced Denaturing (4-20% precut gels)

10 µg

lysis buffer 12h

Nicotiana benthamiana

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 20°C

12 hour(s) and 0 minute(s) · Temperature: 4°C

1/5000

Abcam antibody:Goat Anti-Mouse IgG H&L (HRP)

ECL

2 minute(s) and 0 second(s)

the tubulin should have a band a little bit higher than 5okd, however, no band showed at that position. With the same process, I got very clear GFP band (~27kd).

Thank you for participating in our AbTrial program and your valuable feedback. As the antibody had not yet been tested with this sample type before, we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.