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  1. Link

    alpha-tubulin-antibody-microtubule-marker-ab15246.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Microtubules
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Anti-alpha Tubulin 抗体 - Microtubule Marker (ab15246)

  • Datasheet
  • SDS
Reviews (18) Submit a question References (222)

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Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
  • Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)

Key features and details

  • Rabbit polyclonal to alpha Tubulin - Microtubule Marker
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

こちらの製品もご検討ください

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Recombinant Mouse GAPDH protein (ab202148)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-alpha Tubulin antibody - Microtubule Marker
    alpha Tubulin 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to alpha Tubulin - Microtubule Marker
  • 由来種

    Rabbit
  • 特異性

    Homology to both Tubulin alpha-3E chain and Tubulin alpha-4A chain

  • アプリケーション

    適用あり: WB, IHC-Pmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Pig, Xenopus laevis, Drosophila melanogaster, Zebrafish, Gerbil, Newt
  • 免疫原

    Synthetic peptide within Human alpha Tubulin aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6PEY2

  • 特記事項

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Isotype/Loading Controls
    • Loading Controls
    • Alpha Tubulin
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • SK-N-SH whole cell lysate (ab3956)
  • Related Products

    • alpha Tubulin peptide (ab195584)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab15246の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (14)
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IHC-P (1)
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 配列類似性

    Belongs to the tubulin family.
  • 翻訳後修飾

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 細胞内局在

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68366 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 7277 Human
    • Entrez Gene: 22145 Mouse
    • Entrez Gene: 316531 Rat
    • Entrez Gene: 573122 Zebrafish
    • Entrez Gene: 573216 Zebrafish
    • Omim: 191110 Human
    • SwissProt: P68366 Human
    • SwissProt: P68368 Mouse
    • SwissProt: Q5XIF6 Rat
    • Unigene: 75318 Human
    • Unigene: 1155 Mouse
    • Unigene: 92961 Rat
    see all
  • 別名

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

画像

  • Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
    Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)

    Lanes: 1, 4, 7 - SK N BE; 2, 5, 8 - TE 671; 3, 6, 9 - HeLa

    Lanes: 1-3: 4oC (1 freeze/thaw);  4-6: 4oC (2 freeze/thaws); 7-9 :   4oC (4 freeze/thaws) 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)

    ab15246 at 1/200 dilution, staining alpha Tubulin in Human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin- embedded tissue sections). 

  • Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
    Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
    All lanes : Anti-alpha Tubulin antibody - Microtubule Marker (ab15246) at 1 µg/ml

    Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 50 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (222)

ab15246 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab15246 は 222 報の論文で使用されています。

  • Chen XK  et al. 1-phenyl 2-thiourea (PTU) activates autophagy in zebrafish embryos. Autophagy 17:1222-1231 (2021). PubMed: 32286915
  • Lafouresse F  et al. Stochastic asymmetric repartition of lytic machinery in dividing CD8+ T cells generates heterogeneous killing behavior. Elife 10:N/A (2021). PubMed: 33427199
  • Rashnonejad A  et al. Designed U7 snRNAs inhibit DUX4 expression and improve FSHD-associated outcomes in DUX4 overexpressing cells and FSHD patient myotubes. Mol Ther Nucleic Acids 23:476-486 (2021). PubMed: 33510937
  • Strausfeld N & Sayre ME Shore crabs reveal novel evolutionary attributes of the mushroom body. Elife 10:N/A (2021). PubMed: 33559601
  • Tavares-Valente D  et al. Disruption of pH Dynamics Suppresses Proliferation and Potentiates Doxorubicin Cytotoxicity in Breast Cancer Cells. Pharmaceutics 13:N/A (2021). PubMed: 33572458
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 18 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Swine Testicle (ST) cells infected with pseudorabi)
Permeabilization
Yes - 0.1% Triton-X
Specification
Swine Testicle (ST) cells infected with pseudorabi
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Jul 30 2020

Western blot abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1 cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
THP-1 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Jul 22 2019

Western blot abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10000 cells
Specification
Glioblastoma
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 21°C
Read More

DR. Gilberto Betancor Quintana

Verified customer

投稿 Jun 20 2019

Western blot abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
10 µg
Specification
Heart
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

投稿 Oct 08 2018

Western blot abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Glioblastoma (GL261))
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10000 cells
Treatment
HDAC inhibitors for 24hrs
Specification
Mouse Glioblastoma (GL261)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Read More

Abcam user community

Verified customer

投稿 Sep 12 2014

Western blot abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Non-reduced Denaturing (12.5)
Loading amount
20 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

投稿 May 28 2013

Immunocytochemistry abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (HeLa)
Permeabilization
Yes - triton X-100
Specification
HeLa
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 15°C
Fixative
Formaldehyde
Read More

Ms. AeLee Jeong

Verified customer

投稿 Apr 05 2013

Western blot abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (High-fat diet-fed SD rat muscle)
Gel Running Conditions
Reduced Denaturing (9% SDS-PAGE)
Loading amount
30 µg
Specification
High-fat diet-fed SD rat muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Mar 19 2013

Immunoprecipitation abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293 T)
Total protein in input
100 µg
Immuno-precipitation step
Protein A/G
Specification
HEK 293 T
Read More

Dr. Chris Tracy

Verified customer

投稿 Sep 18 2012

Western blot abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela cells)
Gel Running Conditions
Non-reduced Denaturing (NuPage 4-12% Bis-Tris)
Loading amount
100000 cells
Specification
Hela cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Apr 23 2012

1-10 of 18 Abreviews or Q&A

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