Anti-alpha Tubulin 抗体 [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889)

製品の概要

  • 製品名

    Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)
    alpha Tubulin 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 594)
  • 由来種

    Mouse
  • 標識

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm
  • アプリケーション

    適用あり: ICC/IFmore details
  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Full length native protein (purified) corresponding to Chicken alpha Tubulin.

  • エピトープ

    aa 426-450
  • ポジティブ・コントロール

    • ICC/IF - HeLa
  • 特記事項

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab195889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/150 - 1/250.

ab178000 - Mouse monoclonal IgG1 (Alexa Fluor® 594), is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 配列類似性

    Belongs to the tubulin family.
  • 翻訳後修飾

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 細胞内局在

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース

  • 別名

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

画像

  • ab195889 staining alpha Tubulin in MEF1 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% PBS-Triton X-100 for 5 min then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1hr. The cells were then incubated with ab195889 at 1/250 dilution (shown in orange) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    This product gave a positive signal in 100% methanol (10 min) fixed MEF1 cells under the same testing conditions.

    Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).

  • ab195889 staining alpha Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% PBS-Triton X-100 for 5 min then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1hr. The cells were then incubated with ab195889 at 1/167 dilution (shown in psedo-color red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    This product gave a positive signal in 100% methanol (10 min) fixed HeLa cells under the same testing conditions.

    Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).

参考文献

This product has been referenced in:

  • Qadri MM  et al. cAMP Attenuates TGF-ß's Profibrotic Responses in Osteoarthritic Synoviocytes: Involvement of Hyaluronan and PRG4. Am J Physiol Cell Physiol N/A:N/A (2018). Read more (PubMed: 29898378) »
  • Seifert J  et al. Thrombin-induced cytoskeleton dynamics in spread human platelets observed with fast scanning ion conductance microscopy. Sci Rep 7:4810 (2017). Read more (PubMed: 28684746) »
See all 2 Publications for this product

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