Anti-alpha Tubulin 抗体 [DM1A] - Loading Control (HRP) (ab40742)

製品の概要

  • 製品名

    Anti-alpha Tubulin antibody [DM1A] - Loading Control (HRP)
    alpha Tubulin 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control (HRP)
  • 由来種

    Mouse
  • 標識

    HRP
  • アプリケーション

    適用あり: WB, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Guinea pig, Human, Xenopus laevis
  • 免疫原

    Full length native protein (purified) corresponding to Chicken alpha Tubulin.

  • ポジティブ・コントロール

    • This antibody gave a positive signal in the following: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Brain (Rat) Tissue Lysate - normal tissue Xenopus (Blastula) Whole Cell Lysate. IHC-P: FFPE human colon (normal) tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab40742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 配列類似性

    Belongs to the tubulin family.
  • 翻訳後修飾

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 細胞内局在

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース

  • 別名

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

画像

  • All lanes : Anti-alpha Tubulin antibody [DM1A] - Loading Control (HRP) (ab40742) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 whole cell lysate (ab7179)
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Xenopus Laevis Protein Extracts (Stg 8)

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 57 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes
  • IHC image of alpha Tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab40742, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

参考文献

This product has been referenced in:

  • Kojima ML  et al. Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice. Elife 8:N/A (2019). Read more (PubMed: 30810530) »
  • Wong NKP  et al. Exploring the Roles of CREBRF and TRIM2 in the Regulation of Angiogenesis by High-Density Lipoproteins. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29958463) »
See all 28 Publications for this product

レビューと Q&A

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Baboon Tissue lysate - whole (Fetal Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
12.5 µg
Specification
Fetal Skeletal muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Soon-Ok Kim

Verified customer

投稿 Feb 09 2017

Application
Western blot
Sample
Chicken Tissue lysate - whole (Chicken Embryo)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
Chicken Embryo
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Yuval Cinnamon

Verified customer

投稿 Dec 12 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (melanoma)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
melanoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

投稿 Oct 12 2015

Answer

Thank you and your customer for taking the time to contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody after freezing.
The details your customer has kindly provided will provide us with vital information for our monitoring of product quality.
I appreciate the time your customer has spent in the laboratory and understand their concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there are no tips to provide on this occasion to help improve the results. I can suggest your customer has regrettably received a bad vial.
I apologise for the inconvenience and am pleased to offer your customer a free of charge replacement (also possible with an alternative antibody) or credit note in compensation if the antibody was purchased within the last six month.
Thank you and your customer for your cooperation. I look forward to hearing from you with details of how your customer would like to proceed.

Read More
Application
Western blot
Sample
Rat Cell lysate - whole cell (Neonatal Rat cardiomyocytes)
Loading amount
20 µg
Specification
Neonatal Rat cardiomyocytes
Gel Running Conditions
Reduced Non-Denaturing (Native) (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Mar 30 2011

Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Loading amount
50 µg
Specification
Heart
Gel Running Conditions
Reduced Denaturing (8% Tris/HCL)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Feb 28 2011

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