製品名Anti-alpha Tubulin antibody [DM1A] - Loading Control (Alexa Fluor® 790)
alpha Tubulin 一次抗体 製品一覧
製品の詳細Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control (Alexa Fluor® 790)
標識Alexa Fluor® 790. Ex: 782nm, Em: 805nm
アプリケーション適用あり: WBmore details
種交差性交差種: Mouse, Human
- This antibody gave a positive signal in the following whole cell lysates: HeLa; MCF7; U2OS; NIH3T3.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
バッファーPreservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
精製度Immunogen affinity purified
- Anti-alpha Tubulin antibody [DM1A] - Loading Control (Alexa Fluor® 680) (ab184093)
- Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 488) (ab195887)
- Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889)
- Anti-alpha Tubulin antibody [DM1A] - Loading Control (HRP) (ab40742)
- Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (FITC) (ab64503)
- Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
Our Abpromise guarantee covers the use of ab184577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).|
機能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
配列類似性Belongs to the tubulin family.
翻訳後修飾Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
細胞内局在Cytoplasm > cytoskeleton.
- Information by UniProt
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
All lanes : Anti-alpha Tubulin antibody [DM1A] - Loading Control (Alexa Fluor® 790) (ab184577) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab184577 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx.
ab184577 has not yet been referenced specifically in any publications.