Application
Western blot
Sample
Human Cell lysate - whole cell (Liver)
Gel Running Conditions
Reduced Denaturing (4-12 Bis-tris)
Loading amount
30 µg
Specification
Liver
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBST
Dilution
1/5000
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: Goat anti-Mouse IgG H&L (IRDye® 680RD)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Detection
Detection method
Odyssey
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 52 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 26 2019