Western blot abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control

Good
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (skin tissue)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Treatment
UV B (100mJ/cm2)
Specification
skin tissue
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in TBS
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: anti-mouse IgG
Host species: Human
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
UV B treated (100mJ/cm2)
Exposure
3 second(s)
Bands
Specific: 52 kDa Non-specific: 52 kDa
Positive control
UV B non treated

Abcam user community

Verified customer

投稿 Apr 05 2019

登録