Western blot abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control

Below Average
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Brown adipose tissue)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
50 µg
Specification
Brown adipose tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/4000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: anti-Mouse IgG Dylight 800
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Infrared
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
Positive control
brown adipose tissue

Additional data

Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.

Abcam user community

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投稿 Oct 17 2018

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