Western blot abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (RCC 786-0)
Gel Running Conditions
Reduced Denaturing (4-12% Nupage Bis-Tris)
Loading amount
10 µg
Treatment
Interferon gamma 10ng/ml for 24 hrs
Specification
RCC 786-0
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/10000

Secondary antibody

Dilution
1/10000
Name

Detection

Detection method
ECL+
Exposure
10 second(s)
Bands
Specific: 50 kDa

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投稿 Sep 28 2017

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