Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control

Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (PK15)
Permeabilization
Yes - 1% Triton X-100, 5 mins, Room Temp
Specification
PK15
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200

Secondary antibody

Dilution
1/50
Name
Non-Abcam antibody was used: TRITC CONJUGATE-GOAT ANTI-MOUSE IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: TRITC

Additional data

Additional Notes
Sensitive to cell permeabilisation methods. In controls, methanol/acetone permeabilisation resulted in reduced signal.

Dr. Rob Noad

Verified customer

投稿 Jul 17 2015

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