Western blot abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Daudi (Human Burkitt's lymphoma cell line))
Loading amount
20 µg
Specification
Daudi (Human Burkitt's lymphoma cell line)
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution
1/2500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: non-fat 5% milk

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-mouse IgG-HRP,Santa Cruz biotechnology
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
Thermo scientific super-signal west pico
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
Positive control
Alpha-tubulin was used as a reference protein

Additional data

Additional Notes
Daudi (Human Burkitt's lymphoma cell line) 10, 15 and 20ug load

Dr. Elena Kashuba

Verified customer

投稿 Apr 05 2013

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