Anti-alpha Tubulin 抗体 (ab24246)
Key features and details
- Rabbit polyclonal to alpha Tubulin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-alpha Tubulin antibody
alpha Tubulin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to alpha Tubulin -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide (Human) conjugated to KLH, corresponding to C terminal residues of Human alpha tubulin.
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ポジティブ･コントロール
- HeLa cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.03% Sodium azide
Constituents: HEPES, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
特記事項（精製）
Precipitated by ammonium sulfate. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24246の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000. Predicted molecular weight: 50 kDa.
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| 特記事項 |
|---|
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WB
1/10000. Predicted molecular weight: 50 kDa. |
ターゲット情報
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機能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
配列類似性
Belongs to the tubulin family. -
翻訳後修飾
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 7277 Human
- Omim: 191110 Human
- SwissProt: P68366 Human
- Unigene: 75318 Human
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別名
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
画像
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Western blot - Anti-alpha Tubulin antibody (ab24246)All lanes : Anti-alpha Tubulin antibody (ab24246) at 1/10000 dilution
Lane 1 : HeLa cell lysates (20ug).
Lane 2 : 293T cell lysates (20ug).
Predicted band size: 50 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab24246 は 7 報の論文で使用されています。
- Aghaizu ND et al. Repeated nuclear translocations underlie photoreceptor positioning and lamination of the outer nuclear layer in the mammalian retina. Cell Rep 36:109461 (2021). PubMed: 34348137
- Ponce-Lina R et al. Differential Phosphorylation of the Glucocorticoid Receptor in Hippocampal Subregions Induced by Contextual Fear Conditioning Training. Front Behav Neurosci 14:12 (2020). PubMed: 32116592
- Dumbacher M et al. Modifying Rap1-signalling by targeting Pde6d is neuroprotective in models of Alzheimer's disease. Mol Neurodegener 13:50 (2018). PubMed: 30257685
- Wilson BJ et al. ABCB5 maintains melanoma-initiating cells through a proinflammatory cytokine signaling circuit. Cancer Res 74:4196-207 (2014). PubMed: 24934811
- Kishida M et al. Orexin 2 receptor as a potential target for immunotoxin and antibody-drug conjugate cancer therapy. Oncol Lett 3:525-529 (2012). PubMed: 22740944
- Matsuda Y et al. Nestin is a novel target for suppressing pancreatic cancer cell migration, invasion and metastasis. Cancer Biol Ther 11:512-23 (2011). PubMed: 21258211
- Matsuda Y et al. Morphological and cytoskeletal alterations of nervous system tumor cells with different culturing methods. Int J Oncol 38:1253-8 (2011). PubMed: 21331444