Application
Western blot
Sample
Rat Tissue lysate - whole (High-fat diet-fed SD rat muscle)
Gel Running Conditions
Reduced Denaturing (9% SDS-PAGE)
Loading amount
30 µg
Specification
High-fat diet-fed SD rat muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/2000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG(H+L)-HRP conjugate
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Pico ECL
Exposure
10 second(s)
Bands
Specific: 55 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Mar 19 2013