製品の概要

  • 製品名

    Anti-alpha Tubulin antibody
    alpha Tubulin 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to alpha Tubulin
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC/IF, IHC-Fr, WB, IHC-P, IPmore details
  • 種交差性

    交差種: Mouse, Rat, Sheep, Human, Pig, Drosophila melanogaster, Zebrafish
    交差が予測される動物種: Rabbit, Chicken, Guinea pig, Cow, Xenopus laevis, Gerbil, Newt
  • 免疫原

    Synthetic peptide within Human alpha Tubulin aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6PEY2

  • ポジティブ・コントロール

    • SK N SH whole cell lysate (ab3956) can be used as a positive control in WB. IHC-P: Human lung tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 17785506
IHC-Fr Use at an assay dependent concentration. PubMed: 21069788
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 配列類似性

    Belongs to the tubulin family.
  • 翻訳後修飾

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 細胞内局在

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース

  • 別名

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

画像

  • Lanes: 1, 4, 7 - SK N BE; 2, 5, 8 - TE 671; 3, 6, 9 - HeLa

    Lanes: 1-3: 4oC (1 freeze/thaw);  4-6: 4oC (2 freeze/thaws); 7-9 :   4oC (4 freeze/thaws) 

  • ab15246 at 1/200 dilution, staining alpha Tubulin in Human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin- embedded tissue sections). 

  • This image shows a single confocal z-section of a primary porcine macrophage stained with rabbit polyclonal to alpha tubulin.

    This picture was kindly supplied as part of the review submitted by Peter Delputte (Laboratory of Virology, Ghent University, Belgium).

     

  • All lanes : Anti-alpha Tubulin antibody (ab15246) at 1 µg/ml

    Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 50 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

参考文献

This product has been referenced in:

  • Nardi F  et al. DGAT1 Inhibitor Suppresses Prostate Tumor Growth and Migration by Regulating Intracellular Lipids and Non-Centrosomal MTOC Protein GM130. Sci Rep 9:3035 (2019). Read more (PubMed: 30816200) »
  • Toyoda Y  et al. Functional Characterization of Clinically-Relevant Rare Variants in ABCG2 Identified in a Gout and Hyperuricemia Cohort. Cells 8:N/A (2019). Read more (PubMed: 31003562) »
See all 153 Publications for this product

レビューと Q&A

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1-10 of 17 Abreviews

Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1 cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
THP-1 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Jul 22 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10000 cells
Specification
Glioblastoma
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 21°C

Dr. Gilberto Betancor Quintana

Verified customer

投稿 Jun 20 2019

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
10 µg
Specification
Heart
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

投稿 Oct 08 2018

Application
Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (Mouse Glioblastoma (GL261))
Specification
Mouse Glioblastoma (GL261)
Treatment
HDAC inhibitors for 24hrs
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C

Abcam user community

Verified customer

投稿 Sep 12 2014

Western blot

Excellent
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (12.5)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

投稿 May 28 2013

Application
Immunocytochemistry
Sample
Human Cultured Cells (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 15°C

Ms. AeLee Jeong

Verified customer

投稿 Apr 05 2013

Application
Western blot
Sample
Rat Tissue lysate - whole (High-fat diet-fed SD rat muscle)
Loading amount
30 µg
Specification
High-fat diet-fed SD rat muscle
Gel Running Conditions
Reduced Denaturing (9% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Mar 18 2013

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293 T)
Total protein in input
100 µg
Specification
HEK 293 T
Immuno-precipitation step
Protein A/G

Dr. Chris Tracy

Verified customer

投稿 Sep 18 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (Hela cells)
Loading amount
100000 cells
Specification
Hela cells
Gel Running Conditions
Non-reduced Denaturing (NuPage 4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Apr 23 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293T)
Loading amount
10 µg
Specification
HEK 293T
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Blocking buffer (LiCor, 927-40003) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Jan 20 2012

1-10 of 17 Abreviews

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