Application
Immunocytochemistry/ Immunofluorescence
Blocking step
3% BSA and 0.5% TX100 as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Monkey Cell (Kidney)
Specification
Kidney
Permeabilization
No
Fixative
3% PFA and 0.1% GA
Other product details
Incubation time
45 minute(s) · Temperature: 25°C · Diluent: 3% BSA and 0.5% TX100
Dilution
1/100
Secondary antibody
Concentration
2 µg/ml
Name
Non-Abcam antibody was used: Donkey anti Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Additional data
Additional Notes
Acetylated tubulin is displayed in green. Tyrosinated tubulin was stained with Rat anti Tubulin (YL1/2) antibody and is displayed in magenta. The sample was extracted prior to fixation with 0.5% TX100 in 1x PEM buffer for 20s.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Aaron Halpen
Verified customer
投稿 Sep 29 2014