Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human brain (multiple system atrophy))
Specification
Human brain (multiple system atrophy)
Fixative
Acetone
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 18°C
Other product details
Dilution
1/50
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 18°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Image shows MSA cerebellum.
Abcam response
Many thanks for your feedback on ab10789 (alpha Synuclein antibody). This is a Fast Track antibody that has not been previously tested in IHC or in human tissues. Therefore, it is unclear if this antibody does not work in IHC, human tissue or both. We hope this information is useful and we encourage feedback from our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 24 2011