Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% Gel)
Sample
Human Cell lysate - whole cell (SH-SY5Y Neuroblastoma cell line)
Specification
SH-SY5Y Neuroblastoma cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Bio-rad
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL, Bio rad Chemi-doc
Bands
Specific: 20 kDa Non-specific: 30, 140 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
Improved signal if you wash the membrane in 0,4% PFA in PBS for 30 mins immediately after transfer.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Fredrik Hoel
Verified customer
投稿 Dec 16 2014