Application
Western blot
Sample
Human Cell lysate - whole cell (skmel13, shsy5y)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Specification
skmel13, shsy5y
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: IRDye 800CW
Host species: Sheep
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Sheep
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
chemidoc
Negative control
SNCA KO cells
Exposure
30 second(s)
Bands
Specific: 15 kDa
Positive control
WT Cells
Additional data
Additional Notes
Dilution rate is very high but one could get good bands using this antibody. Better for overnight incubations. You would get non specific bands at higher molecular weights.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 17 2021