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  1. Link

    alpha-synuclein-antibody-ab6162.pdf

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Neuroscience Neurology process Neurodegenerative disease Parkinson's disease Synuclein
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Anti-Alpha-synuclein 抗体 (ab6162)

  • Datasheet
  • SDS
Reviews (4)Q&A (5)References (22)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Alpha-synuclein antibody (ab6162)

    Key features and details

    • Sheep polyclonal to Alpha-synuclein
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    こちらの製品もご検討ください

    タンパク質
    Product image
    Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)
    二次抗体
    Product image
    Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)

    関連製品

    製品の概要

    • 製品名

      Anti-Alpha-synuclein antibody
      Alpha-synuclein 一次抗体 製品一覧
    • 製品の詳細

      Sheep polyclonal to Alpha-synuclein
    • 由来種

      Sheep
    • 特異性

      Human and rat alpha synuclein. Cross reactivity: This antibody is known to react with human, mouse, rat and other rodents. Cross reactivity with other species has not yet been tested.
    • アプリケーション

      適用あり: WBmore details
      適用なし: Flow Cyt or ICC/IF
    • 種交差性

      交差種: Mouse, Rat, Human
    • 免疫原

      Synthetic peptide corresponding to Human Alpha-synuclein aa 116-131. Synthetic peptide of human synuclein (116-131) coupled to diphtheria toxoid with cysteine residue attached to N-ter of peptide. Conjugate cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC).
      Sequence:

      C-MPVDPDNEAYEMPSEE

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      pH: 7
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Parkinson's disease
      • Synuclein
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Tangles & Tau

    関連製品

    • Isotype control

      • Sheep IgG - Isotype Control (ab37385)
    • Recombinant Protein

      • Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)
      • Recombinant Human Alpha-synuclein (mutated A53T) protein (ab51184)
      • Recombinant Human Alpha-synuclein protein (ab51189)

    アプリケーション

    Our Abpromise guarantee covers the use of ab6162 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use a concentration of 0.5 µg/ml.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC/IF.
  • ターゲット情報

    • 機能

      May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
    • 組織特異性

      Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
    • 関連疾患

      Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
      Parkinson disease 1
      Parkinson disease 4
      Dementia Lewy body
    • 配列類似性

      Belongs to the synuclein family.
    • ドメイン

      The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
    • 翻訳後修飾

      Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
      Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
      Ubiquitinated. The predominant conjugate is the diubiquitinated form.
      Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
    • 細胞内局在

      Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
    • Target information above from: UniProt accession P37840 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 6622 Human
      • Entrez Gene: 20617 Mouse
      • Entrez Gene: 29219 Rat
      • Omim: 163890 Human
      • SwissProt: P37840 Human
      • SwissProt: O55042 Mouse
      • SwissProt: P37377 Rat
      • Unigene: 21374 Human
      • Unigene: 17484 Mouse
      • Unigene: 1827 Rat
      see all
    • 別名

      • Alpha synuclein antibody
      • Alpha-synuclein antibody
      • Alpha-synuclein, isoform NACP140 antibody
      • alphaSYN antibody
      • MGC105443 antibody
      • MGC110988 antibody
      • MGC127560 antibody
      • MGC64356 antibody
      • NACP antibody
      • Non A beta component of AD amyloid antibody
      • Non A4 component of amyloid antibody
      • Non A4 component of amyloid precursor antibody
      • Non-A beta component of AD amyloid antibody
      • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
      • Non-A4 component of amyloid precursor antibody
      • Non-A4 component of amyloid, precursor of antibody
      • OTTHUMP00000218549 antibody
      • OTTHUMP00000218551 antibody
      • OTTHUMP00000218552 antibody
      • OTTHUMP00000218553 antibody
      • OTTHUMP00000218554 antibody
      • PARK 1 antibody
      • PARK 4 antibody
      • PARK1 antibody
      • PARK4 antibody
      • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
      • Parkinson disease familial 1 antibody
      • SNCA antibody
      • Snca synuclein antibody
      • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYN antibody
      • Synuclein alpha antibody
      • Synuclein alpha 140 antibody
      • Synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYUA_HUMAN antibody
      see all

    画像

    • Western blot - Anti-Alpha-synuclein antibody (ab6162)
      Western blot - Anti-Alpha-synuclein antibody (ab6162)
      All lanes : Anti-Alpha-synuclein antibody (ab6162) at 1 µg/ml

      Lane 1 : Recombinant Human a -synuclein at 0.1 µg
      Lane 2 : Mouse brain lysate at 30 µg

      Secondary
      All lanes : anti-sheep HRP at 1/6000 dilution

    プロトコール

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (22)

    ab6162 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6162 は 22 報の論文で使用されています。

    • Fragniere AMC  et al. Hyperosmotic stress induces cell-dependent aggregation of a-synuclein. Sci Rep 9:2288 (2019). PubMed: 30783136
    • Han C  et al. Exosomes from patients with Parkinson's disease are pathological in mice. J Mol Med (Berl) 97:1329-1344 (2019). PubMed: 31302715
    • Alarcón-Arís D  et al. Selective a-Synuclein Knockdown in Monoamine Neurons by Intranasal Oligonucleotide Delivery: Potential Therapy for Parkinson's Disease. Mol Ther 26:550-567 (2018). PubMed: 29273501
    • Svarcbahs R  et al. Removal of prolyl oligopeptidase reduces alpha-synuclein toxicity in cells and in vivo. Sci Rep 8:1552 (2018). PubMed: 29367610
    • Lautenschläger J  et al. C-terminal calcium binding of a-synuclein modulates synaptic vesicle interaction. Nat Commun 9:712 (2018). PubMed: 29459792
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-9 of 9 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Alpha-synuclein antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 23 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Synuclein antibody

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (synaptosomes isolated from rat brain)
    Permeabilization
    Yes - 0.05 % Tween 20
    Specification
    synaptosomes isolated from rat brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 24 2015

    Question

    What is in the buffer in the antibody before lyophilisation?

    Read More

    Abcam community

    Verified customer

    Asked on May 10 2012

    Answer

    Thank you for calling Abcam yesterday.

    I have heard back from the lab about the buffer of ab6162. All of our affinity purified antibody ultimately end up in PBS from which they are then lyophilized, thus all that is remaining are some buffer salts and antibody protein once dried. Initial composition was 50mM Na+Phosphate, 150mM NaCL, final pH 7.5. There are no preservatives.

    I hope this helps, please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    Answered on May 10 2012

    Question

    Now I need information about IHC Kit for rat. I read abcam catalog,but I not foundspecificfor rat. My primer antibody is sheep polyclonal of alpha synuclein ( ab 6162) and this antibody will be used for IHC-P for brain rat. Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 10 2012

    Answer

    Thank you very much for your enquiry.

    Unfortunately, we currently do not have a particular ready-to-useIHC Kit available that has been optimised for the use of a sheep polyclonal primary antibody with rat tissue.

    Therefore, if you do not have the appropriate components available,I may recommend the individual components for IHC which are listed in more detail at https://www.abcam.com/index.html?pageconfig=resource&rid=12853.

    For example, I would suggest one of the secondary antibodiesab6747 or ab97125:
    https://www.abcam.com/index.html?datasheet=6747 (or use the following: https://www.abcam.com/index.html?datasheet=6747).
    https://www.abcam.com/index.html?datasheet=97125 (or use the following: https://www.abcam.com/index.html?datasheet=97125).

    As you may see, we also have
    buffers (https://www.abcam.com/index.html?pageconfig=resource&rid=12854),
    diluents (https://www.abcam.com/index.html?pageconfig=resource&rid=12864),
    blocking solutions/serum (https://www.abcam.com/index.html?pageconfig=resource&rid=12859or https://www.abcam.com/index.html?pageconfig=resource&rid=12889)
    and substrates (https://www.abcam.com/index.html?pageconfig=resource&rid=12908or https://www.abcam.com/index.html?pageconfig=resource&rid=12886)
    for you to tailor your specific experiments with rat brain sectionsavailable.

    These include for example the following products:

    ab64214: https://www.abcam.com/index.html?datasheet=64214 (or use the following: https://www.abcam.com/index.html?datasheet=64214).

    ab64216: https://www.abcam.com/index.html?datasheet=64216 (or use the following: https://www.abcam.com/index.html?datasheet=64216).

    ab64211: https://www.abcam.com/index.html?datasheet=64211 (or use the following: https://www.abcam.com/index.html?datasheet=64211).

    ab64226: https://www.abcam.com/index.html?datasheet=64226 (or use the following: https://www.abcam.com/index.html?datasheet=64226).

    ab64238: https://www.abcam.com/index.html?datasheet=64238 (or use the following: https://www.abcam.com/index.html?datasheet=64238).

    Also, I have attached our protocols for your consideration.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Apr 10 2012

    Question

    Has the production or formulation of this antibody changed at all since 2009?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 12 2011

    Answer

    The production and formulation of this sheep antibody to alpha synuclein has not changed since 2009. I hope this is helpful. Please contact us again if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Sep 12 2011

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-alpha Synuclein antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Alpha Synuclein Transgenic Mouse brain)
    Specification
    Alpha Synuclein Transgenic Mouse brain
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.3% TritonX in 0.1% PBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    投稿 May 18 2011

    Immunohistochemistry (Frozen sections) abreview for Anti-alpha Synuclein antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: Vector ABC
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 21 2006

    Question

    I ordered this antibody after I was advised that it worked very well on parafin sections at a concentration of 1/500 to 1/1000. I was wondering if a pretreatment was used, if so which one and what control tissue was used? I've used concentrations ranging from 1/100 to 1/1000 on normal and diseased brain, with and without a pretreatment of microwaving....all my immuno so far has been negative!!!

    Read More

    Abcam community

    Verified customer

    Asked on Aug 08 2001

    Answer

    We assume you are staining for Lewy bodies in human diseased brains. We are using this antibody every week. The staining of Lewy bodies is really quite superb, either in paraffin or cryostat sections. The human tissue is fixed in Zamboni's fixative (4% formaldehyde with 15% saturated picric acid) and used without antigen retrieval. Occassionally we have found some minor improvement using antigen retrieval in 0.1M citrate buffer pH 6.0 improves staining but we rarely use it as staining is always clear.

    Read More

    Jonathan Milner

    Abcam Scientific Support

    Answered on Aug 09 2001

    Question

    Can the antibody be used on parafin blocks? Doesn't state this on the datasheet.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 26 2001

    Answer

    This antibody works very well on paraffin sections. The antibody will need to be used at a concentration a little higher than for cryostat sections, about 1/500 to 1/1000.

    Read More

    Neil Hayward

    Abcam Scientific Support

    Answered on Jul 30 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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