Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Notophthalmus viridescens Tissue sections (Eye)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
Yes - PBST
Specification
Eye
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% Serum in PBST
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: IgG (H+L) Highly Cross-Adsorbed Donkey anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Additional data
Additional Notes
Figure legend:
A)Anterior eye (ciliary muscle) 8 days after lens removal, 10x magnification
B)Anterior eye (ciliary muscle) 8 days after lens removal, 40x magnification without DAPI
C)Anterior eye (ciliary muscle) 8 days after lens removal without primary antibody, 10x magnification
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Georgios Tsissios
Verified customer
投稿 Apr 28 2021