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Anti-alpha smooth muscle Actin 抗体 (ab5694)

  • Datasheet
  • SDS
Reviews (143)Q&A (14)References (1683)

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Abpromise

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Western blot - Anti-alpha smooth muscle Actin antibody (ab5694)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
  • Western blot - Anti-alpha smooth muscle Actin antibody (ab5694)

Key features and details

  • Rabbit polyclonal to alpha smooth muscle Actin
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-alpha smooth muscle Actin antibody [EPR5368] (ab124964)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-alpha smooth muscle Actin antibody
    alpha smooth muscle Actin 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to alpha smooth muscle Actin
  • 由来種

    Rabbit
  • 特異性

    Alpha smooth muscle actin antibody (ab5694) stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. ab5694 reacts with tumors arising from smooth muscles and myoepithelial cells. The other actins, such as beta- and gamma-cytoplasmic, striated muscle and myocardium are not stained by this alpha smooth muscle Actin antibody.

  • アプリケーション

    適用あり: WB, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human alpha smooth muscle Actin (N terminal).
    Database link: P62736

  • ポジティブ・コントロール

    • WB: HEK-293, A431, HeLa, Jurkat and NIH/3T3 whole cell lysate. Mouse heart tissue homogenate. HeLa nuclear lysate. IHC-P: PACT-sRIMS-cleared virgin and lactating mammary glands. Mouse intestine and pancreas tissue. Mouse aortic regurgitation. Mouse tonsil tissue.
  • 特記事項

    Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation and altered by pathological conditions including oncogenic transformation and atherosclerosis.

    Abcam is committed to meeting high standards of manufacturing and has decided to discontinue this product once the stock runs out as we are unable to secure its future high-quality supply. We suggest ab124964, ab150301 or ab7817 as possible replacements. We are sorry for any inconvenience this may cause.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins
    • Cardiovascular
    • Vasculature
    • Vasculature Markers
    • Arterial
    • Cardiovascular
    • Vasculature
    • Smooth muscle cell (SMC)
    • Cytoskeleton
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Smooth Muscle Cells

関連製品

  • Alternative Versions

    • Anti-Vimentin antibody [LN-6] (ab230171)
    • Anti-Vimentin antibody [LN-6] - BSA and Azide free (ab269571)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • Jurkat whole cell lysate (ab7899)
    • A431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human alpha smooth muscle Actin protein (ab134555)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Phalloidin-iFluor 488 Reagent (ab176753)
    • Phalloidin-iFluor 647 Reagent (ab176759)
    • Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)
    • Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

アプリケーション

Our Abpromise guarantee covers the use of ab5694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 関連疾患

    Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • 配列類似性

    Belongs to the actin family.
  • 細胞内局在

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P62736 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 59 Human
    • Entrez Gene: 11475 Mouse
    • Omim: 102620 Human
    • SwissProt: P62736 Human
    • SwissProt: P62737 Mouse
    • Unigene: 500483 Human
    • Unigene: 213025 Mouse
    • 別名

      • a actin antibody
      • AAT6 antibody
      • ACTA_HUMAN antibody
      • ACTA2 antibody
      • Actin alpha 2 smooth muscle aorta antibody
      • Actin aortic smooth muscle antibody
      • Actin, aortic smooth muscle antibody
      • ACTSA antibody
      • ACTVS antibody
      • Alpha 2 actin antibody
      • Alpha actin 2 antibody
      • Alpha cardiac actin antibody
      • alpha sma antibody
      • Alpha-actin-2 antibody
      • Cell growth inhibiting gene 46 protein antibody
      • Cell growth-inhibiting gene 46 protein antibody
      • GIG46 antibody
      • Growth inhibiting gene 46 antibody
      • MYMY5 antibody
      see all

    画像

    • Western blot - Anti-alpha smooth muscle Actin antibody (ab5694)
      Western blot - Anti-alpha smooth muscle Actin antibody (ab5694)
      Lanes 1-3 : Anti-alpha smooth muscle Actin antibody (ab5694) at 1 µg/ml


      Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate - overexpressing alpha-Actin
      Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) cell lysate
      Lanes 3 & 5 : Mouse heart tissue homogenate
      Lane 4 : NIH/3T3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Fluor 750-conjugated goat anti-rabbit IgG (H+L) at 1/12500 dilution

      Predicted band size: 42 kDa
      Observed band size: 42 kDa



      Incubated with the primary antibody at 4°C overnight.

      Incubated with the secondary antibody at room temperature for 1 hour.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)Image from Lloyd-Lewis B. et al., Breast Cancer Res. 2016 18: 127. Fig 2.; doi: 10.1186/s13058-016-0754-9. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

      Passive CLARITY technique (PACT)-sorbitol refractive index matching solution (sRIMS) clearing and 3D imaging of virgin and lactating mouse mammary tissue. a PACT-sRIMS tissue clearing and immunostaining protocol and timeline. Three-dimensional confocal imaging of PACT-sRIMS-cleared virgin (b) and lactating (c) mammary glands immunostained with basal cell markers (K5 and smooth muscle actin (stained with ab5694)) and luminal cell markers (K8 and E-cadherin (E-CAD)). Main image shows the maximum intensity projection of the entire image sequence, with thin optical slices (1 μm) and their depth (z value) relative to the first image in the image sequence.

      From a paper comparing imaging of intact virgin and lactating mammary glands using 3D imaging of solvent-cleared organs, see deep brain (seeDB), clear unobstructed brain imaging cocktails (CUBIC) and passive clarity technique.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)

      This picture shows formalin-fixed, paraffin embedded mouse intestine and mesentery, the optimal dilution is 1:1600 to 1:3200, incubation overnight at 4oC, counterstained with Hematoxylin.

      This image was kindly supplied as part of the review by JQ Zhang.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)Image from Lee H. et al., BMC Dev Biol. 2014 Dec 21;14:48. Fig 2Bdoi: 10.1186/s12861-014-0048-3 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

      Pancreatic vessel imaging in the intact adult mouse pancreas. In adult mouse tissues (12 weeks old), imaging was performed after CLARITY. Three-dimensional projection clarified mouse pancreas with capillary immunostained for α-smooth muscle actin (green). Scale bar, 200 μm.

      From a study, that aimed to improve the original CLARITY procedure and developed specific CLARITY protocols for various intact organs.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)Freytsis, M. et al PLoS One. 2018 Jan 5;13(1):e0190623. doi: 10.1371/journal.pone.0190623. eCollection 2018 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Representative histology of aortic valve leaflets from aged mice demonstrates changes in pRb cKO AoV

      A) Masson’s trichrome showing reduced collagen staining (blue) in leaflet from pRb cKO mouse with aortic regurgitation (AR). B) Movat pentachrome showing more diffuse collage staining (yellow) in fibrosa, but normal proteoglycan staining (blue) in the spongiosa layer of the leaflet from pRb cKO with AR. C) Immunohistochemistry for α-SMA, demonstrating presence of activated myofibroblasts throughout leaflets of pRb cKO mouse with and without AR. Scale bar is 50μm.

      Alpha smooth muscle Actin is detected witn ab5694 at 1/1000 dilution.

      (From Figure 2 of Freytsis et al)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling alpha smooth muscle Actin with ab5694 at a dilution of 1/1000. Heat mediated antigen retrieval was performed for 35 minutes followed by cooling for 20 minutes. Sections were incubated with the primary antibody for 1 hour followed by incubation with a biotinylated secondary antibody for 30 minutes then HRP-Streptavidin for 30 minutes. Developed using DAB chromogen substrate (5-10 minutes). Counter stained with hematoxylin.

      Magnification: left - 10X, right - 40X.

    • Western blot - Anti-alpha smooth muscle Actin antibody (ab5694)
      Western blot - Anti-alpha smooth muscle Actin antibody (ab5694)
      All lanes : Anti-alpha smooth muscle Actin antibody (ab5694) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear cell lysate
      Lane 2 : HeLa whole cell lysate
      Lane 3 : A431 (Human epidermoid carcinoma cell line) cell lysate
      Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
      Lane 5 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Alexa Fluor anti-rabbit at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size: 42 kDa
      Observed band size: 42 kDa
      Additional bands at: 30 kDa, 35 kDa, 37 kDa, 50 kDa, 75 kDa. We are unsure as to the identity of these extra bands.



      Please note that ab5694 does not appear to be specific to smooth muscle.

    プロトコール

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (1683)

    ab5694 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5694 は 1683 報の論文で使用されています。

    • Wu BM  et al. Margatoxin mitigates CCl4-induced hepatic fibrosis in mice via macrophage polarization, cytokine secretion and STAT signaling. Int J Mol Med 45:103-114 (2020). PubMed: 31746414
    • Maniati E  et al. Mouse Ovarian Cancer Models Recapitulate the Human Tumor Microenvironment and Patient Response to Treatment. Cell Rep 30:525-540.e7 (2020). PubMed: 31940494
    • Zhang NN  et al. Flavanomarein inhibits high glucose-stimulated epithelial-mesenchymal transition in HK-2 cells via targeting spleen tyrosine kinase. Sci Rep 10:439 (2020). PubMed: 31949205
    • Buniatian GH  et al. Antifibrotic Effects of Amyloid-Beta and Its Loss in Cirrhotic Liver. Cells 9:N/A (2020). PubMed: 32089540
    • Xie S  et al. Andrographolide Protects Against Adverse Cardiac Remodeling After Myocardial Infarction through Enhancing Nrf2 Signaling Pathway. Int J Biol Sci 16:12-26 (2020). PubMed: 31892842
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 157 Abreviews or Q&A

    Question

    I'm ordering two antibodies (Anti-alpha smooth muscle Actin antibody and collagen III) for rabbit fibroblast. My tissue is rabbit conjunctiva, and I am planning to use the tissue samples for immunochemical staining. We place an implant under the conjunctiva and connect it to the rabbit anterior chamber. We are interested in examining the fibroblasts specifically, as well as any scar tissue that is present. When choosing between ab5694, 18147 and 115944, do you have any suggestions as to which would work the best for HRP detection?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 27 2012

    Answer

    I have checked the website and the datasheets of these three antibodies and it seems that only ab18147 has been tested for cross-reactivity with rabbit. The other two antibodies are not known yet if they recognize rabbit alpha smooth muscle Actin.

    However, it is important to mention that according to the datasheet ab18147 recognizes the alpha-smooth muscle isoform of actin but it does not react with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial).

    We have a nice range of products for detection (ie. anti-mouse HRP based detection kits and reagents), you may wish to visit this site to get some further information:

    https://www.abcam.com/index.html

    Read More

    Abcam Scientific Support

    Answered on Apr 27 2012

    Question

    Has the shelf life for AB5694 been changed? A customer has bought this antibody some years ago from you and then the shelf life was several years. Now she has bought the antibody again and the shelf life is much shorter now. Why the change?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 05 2012

    Answer

    We do not have set expiration dates for our products. I am therefore also not aware that the information on the shelf life was changed. We indeed increased the length of our Abpromise guarantee to six month instead of 120 days.

    I can confirm that most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased.

    These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least six months from the date of purchase when stored correctly.

    For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols).

    Read More

    Abcam Scientific Support

    Answered on Mar 05 2012

    Question

    Non-specific staining of actin filaments in epithelial cells was observed. Do you have any suggestions?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 22 2012

    Answer

    I have some information which may be of interest to you in regards to anti-alpha smooth muscle Actin antibody (ab5694).

    I have found reports of alpha-SMA expression in endothelial cells. These can be found from the links below:

    http://www.ncbi.nlm.nih.gov/pubmed/19285011

    http://www.ncbi.nlm.nih.gov/pubmed/10330261

    http://www.ncbi.nlm.nih.gov/pubmed/15588509

    It may therefore be that the band you are seeing is stillspecificfor smooth muscle but expressed in the epithelial layer.

    Read More

    Abcam Scientific Support

    Answered on Feb 22 2012

    IHC - Wholemount abreview for Anti-alpha smooth muscle Actin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Human Embryo (kidney)
    Specification
    kidney
    Read More

    Dr. Alain Chedotal

    Verified customer

    投稿 Nov 22 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha smooth muscle Actin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Colon)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Colon
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More

    Mr. Victor Bernal

    Verified customer

    投稿 Aug 28 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-alpha smooth muscle Actin antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (Heart)
    Permeabilization
    Yes - Triton
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 25 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha smooth muscle Actin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (heart)
    Permeabilization
    Yes - 0.5% PBST
    Specification
    heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 17 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha smooth muscle Actin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (TAA-induced NASH liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    Yes - 3% H2O2
    Specification
    TAA-induced NASH liver
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 24 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha smooth muscle Actin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Sheep Tissue sections (Ovarian tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: high pH buffer DAKO
    Permeabilization
    No
    Specification
    Ovarian tissue
    Blocking step
    peroxidase block as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Danielle Calado

    Verified customer

    投稿 Oct 14 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha smooth muscle Actin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Dentin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Vetor Unmasking solution
    Permeabilization
    Yes - 0.05% triton-x100
    Specification
    Dentin
    Blocking step
    Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 19 2019

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