Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Epithelial (hTert-RPE1))
Permeabilization
No
Specification
Epithelial (hTert-RPE1)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Methanol
Other product details
Incubation time
1 hour(s) and 0 minute(s)
Dilution
1/250
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Donkey anti Goat, F(ab')2 fragment, cross-absorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Additional data
Additional Notes
Picture: green: beta Tubulin, red: anti alpha SMA.
Note: On the same cells fixed with PAF 4% plus permeabilisation 0.1% Triton x100 10 min, the antibody didn't work. It gave a signal that could be a staining of the Golgi apparatus.
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投稿 May 23 2017