Anti-alpha smooth muscle Actin 抗体 (ab21027)
Key features and details
- Goat polyclonal to alpha smooth muscle Actin
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-alpha smooth muscle Actin antibody
alpha smooth muscle Actin 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to alpha smooth muscle Actin -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Dog, Pig
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免疫原
Synthetic peptide corresponding to Human alpha smooth muscle Actin aa 1-100 (N terminal).
Database link: P62736 -
ポジティブ･コントロール
- WB: Human duodenum lysate. HeLa and NIH/3T3 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21027の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
A 1 hour primary incubation at room temperature is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa). A 1 hour primary incubation at room temperature is recommended for this product. |
ターゲット情報
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機能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
関連疾患
Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. -
配列類似性
Belongs to the actin family. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 477587 Dog
- Entrez Gene: 59 Human
- Entrez Gene: 11475 Mouse
- Entrez Gene: 733615 Pig
- Entrez Gene: 81633 Rat
- Omim: 102620 Human
- SwissProt: P62736 Human
- SwissProt: P62737 Mouse
see all -
別名
- a actin antibody
- AAT6 antibody
- ACTA_HUMAN antibody
see all
画像
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Western blot - Anti-alpha smooth muscle Actin antibody (ab21027)All lanes : Anti-alpha smooth muscle Actin antibody (ab21027) at 1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 42 kDaLysates in RIPA buffer.
Detected by chemiluminescence.
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Western blot - Anti-alpha smooth muscle Actin antibody (ab21027)Anti-alpha smooth muscle Actin antibody (ab21027) at 1 µg/ml + human duodenum lysate at 35 µg
Predicted band size: 42 kDaLysates in RIPA buffer.
Detected by chemiluminescence.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (108)
ab21027 は 108 報の論文で使用されています。
- Chen JY et al. The influence of substrate stiffness on osteogenesis of vascular smooth muscle cells. Colloids Surf B Biointerfaces 197:111388 (2021). PubMed: 33096417
- Min J et al. Phenotype and biological characteristics of endometrial mesenchymal stem/stromal cells: A comparison between intrauterine adhesion patients and healthy women. Am J Reprod Immunol 85:e13379 (2021). PubMed: 33206449
- Chahla J et al. Assessing the Resident Progenitor Cell Population and the Vascularity of the Adult Human Meniscus. Arthroscopy 37:252-265 (2021). PubMed: 32979500
- Bagdadi N et al. The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33498962
- Jeon HH et al. Clinical application of a FOXO1 inhibitor improves connective tissue healing in a diabetic minipig model. Am J Transl Res 13:781-791 (2021). PubMed: 33594326