Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (IMR-90 (Human Lung Fibroblast Cell Line))
Permeabilization
Yes - 0.1% TritonX-100
Specification
IMR-90 (Human Lung Fibroblast Cell Line)
Blocking step
Cas-Block (Invitrogen) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Antibody Diluent (Invitrogen)
Dilution
1/200
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: anti Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 14 2017