Application
Western blot
Sample
Rat Cell lysate - whole cell (Neonatal Cardiac Myocytes)
Loading amount
40 µg
Specification
Neonatal Cardiac Myocytes
Treatment
phenylephrine for 24 hours
Gel Running Conditions
Reduced Denaturing (10% polyacrylamide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% milk in PBST
Secondary antibody
Name
Non-Abcam antibody was used: donkey anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Donkey
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/1000
Detection
Detection method
ECL
Exposure
1 second(s)
Bands
Specific: appox 43 kDa
Additional data
Additional Notes
This antibody recognizes a clean band of the correct size with virtually no background.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Richard Patten
Verified customer
投稿 Jun 02 2008