alpha-L-Fucosidase Assay Kit (ab272525)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Cell Lysate, Plasma, Serum, Tissue Lysate
製品の概要
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製品名
alpha-L-Fucosidase Assay Kit -
検出方法
Colorimetric -
サンプルの種類
Serum, Plasma, Cell Lysate, Tissue Lysate -
アッセイタイプ
Quantitative -
検出範囲
1 mU/ml - 100 mU/ml -
製品の概要
alpha-L-Fucosidase Assay Kit (ab272525) is a non-radioactive, colorimetric assay based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.
High sensitivity and wide linear range. Linear detection range (10 µL sample): 1 to 100 U/L for a 20 minute reaction.
Homogeneous and simple procedure. Simple add-mix-read procedure allows reliable quantitation of fucosidase activity within 20 minutes.
Robust and amenable to HTS. All reagents are compatible with highthroughput liquid handling instruments. Can be readily automated to measure thousands of samples per day.
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アプリケーション
適用あり: Functional Studiesmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 100 tests Standard (12.5 mM Nitrophenol) 1 x 1ml Stop Reagent 1 x 12ml Substrate Buffer 1 x 10ml -
研究分野
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細胞内局在
FUCA1: Lysosome. FUCA2: Secreted.
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 特記事項 |
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|
Functional Studies
Use at an assay dependent concentration. |
画像
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Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example Standard Curve.
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Activity Titration CurveExample activity titration curve.
参考文献 (0)
ab272525 は論文での使用が確認できていません。
