Key features and details
- Mouse monoclonal [2E3] to alpha Internexin
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Rat, Cow, Human
- Isotype: IgG1
製品名Anti-alpha Internexin antibody [2E3]
alpha Internexin 一次抗体 製品一覧
製品の詳細Mouse monoclonal [2E3] to alpha Internexin
特異性specifically recognizes a-internexin.
アプリケーション適用あり: WB, Flow Cyt, ICC/IFmore details
種交差性交差種: Rat, Cow, Human
Recombinant full length protein corresponding to Rat alpha Internexin. (Purified from E. coli).
エピトープC-terminal non-helical extension of the protein.
- WB: Rat and mouse brain and spinal cord lysates; Cow spinal cord lysate. Flow Cyt: SH-SY5Y cells.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.03% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
精製度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab7654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100. Predicted molecular weight: 66 kDa.|
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
機能Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
組織特異性Found predominantly in adult CNS.
配列類似性Belongs to the intermediate filament family.
発生段階Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 66 kDa neurofilament protein antibody
- AINX_HUMAN antibody
- Alpha Inx antibody
All lanes : Anti-alpha Internexin antibody [2E3] (ab7654) at 1/10000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat spinal cord lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse spinal cord lysate
Lane 5 : Cow spinal cord lysate
Predicted band size: 66 kDa
Hippocampal neurons in tissue culture with neurofilament alpha internexin (red) (Ab7654) and GFAP (green). Photo courtesy of: Dr. Gerry Shaw University of Florida
All lanes : Anti-alpha Internexin antibody [2E3] (ab7654)
Lanes 1-2 : Crude homogenate of rat facial nucleus at 3 days after experimental injury
Lanes 3-4 : Crude homogenate of rat facial nucleus at 5 days after experimental injury
Lanes 5-6 : Crude homogenate of rat facial nucleus at 7 days after experimental injury
Lanes 7-8 : Crude homogenate of rat facial nucleus at 14 days after experimental injury
Lanes 9-10 : Crude homogenate of rat facial nucleus at 28 days after experimental injury
Predicted band size: 66 kDa
Observed band size: 66 kDa
Overlay histogram showing SH-SY5Y cells stained with ab7654 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7654, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab7654 は 3 報の論文で使用されています。
- Rauch U et al. Expression of intermediate filament proteins and neuronal markers in the human fetal gut. J Histochem Cytochem 54:39-46 (2006). PubMed: 16087706
- Uchikado H et al. Screening for neurofilament inclusion disease using alpha-internexin immunohistochemistry. Neurology 64:1658-9 (2005). PubMed: 15883343
- Ko TL et al. The expression of alpha-internexin and peripherin in the developing mouse pineal gland. J Biomed Sci 12:777-89 (2005). PubMed: 16132113