製品の概要

  • 製品名

    Alpha-Glucosidase Activity Assay Kit (Colorimetric)
    alpha-glucosidase キット 製品一覧
  • 検出方法

    Colorimetric
  • サンプルの種類

    Saliva, Serum, Cell culture extracts, Tissue, Adherent cells, Suspension cells
  • アッセイタイプ

    Enzyme activity (quantitative)
  • 検出範囲

    0.1 mU/well - 10 mU/well
  • 種交差性

    交差種: Mammals, Other species
  • 製品の概要

    In Abcam's Alpha-Glucosidase Activity Assay Kit (Colorimetric) (ab174093), α-Glucosidase hydrolyzes the Substrate Mix to release the p-nitrophenol that can be measured colorimetrically (OD = 410 nm). This is an easy, quick and high-throughput capable kit that can measure 0.1-10 mU of α-glucosidase activity in a variety of samples.

     

    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    α-Glucosidase breaks down α-1,4 linked polysaccharides to glucose, which can be utilized as a source of energy. In the biotechnology industry, α-glucosidase is used to produce glucose from intermediate breakdown products of starch hydrolysis generated by enzymes such as amylase.

    Pompe disease, one of the 12 known glycogen storage diseases, is an autosomal recessive metabolic disorder attributed to α-glucosidase deficiency. In this disease, glycogen accumulates in the lysosomes, resulting in progressive muscle weakness, heart failure and other neurological symptoms.

  • 試験プラットフォーム

    Microplate reader

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 ラベル 100 tests
    Alpha Glucosidase Assay Buffer WM 1 x 25ml
    Alpha Glucosidase Positive Control 1 vial
    Alpha Glucosidase Substrate Mix Amber 1 x 0.3ml
    p-Nitrophenol Standard (100 mM) 1 x 100µl
  • 研究分野

  • 関連性

    a-Glucosidase breaks down a-1,4 linked polysaccharides to glucose, which can be utilized as a source of energy. In the biotechnology industry, a-glucosidase is used to produce glucose from intermediate breakdown products of starch hydrolysis generated by enzymes such as amylase. Pompe disease, one of the 12 known glycogen storage diseases, is an autosomal recessive metabolic disorder attributed to a- glucosidase deficiency. In this disease, glycogen accumulates in the lysosomes, resulting in progressive muscle weakness, heart failure and other neurological symptoms.
  • 細胞内局在

    Lysosome. Lysosome membrane.
  • 別名

    • Acid Maltase
    • Aglucosidase Alfa
    • EC 3.2.1.20
    • Glucosidase, Alpha; Acid
    • LYAG
    • Lysosomal Alpha-Glucosidase
    see all

画像

  • Time course of Alpha-Glucosidase Activity in mouse liver tissue lysate

  • Time course of Alpha-Glucosidase Activity in various samples

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Kinetic profile of various amounts (0, 2, 4, 6, 8 & 10 mU) of α-glucosidase run at 25°C under this protocol. Inset: Results for 0-0.2-0.4-0.6-0.8-1.0 mU of α-glucosidase. Data points after 5 minutes were used to determine slope. This is example data only.

プロトコール

参考文献

ab174093 has not yet been referenced specifically in any publications.

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