Alpha-Glucosidase Activity Assay Kit (Colorimetric) (ab174093)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Cell culture extracts, Saliva, Serum, Suspension cells, Tissue
製品の概要
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製品名
Alpha-Glucosidase Activity Assay Kit (Colorimetric)
alpha-glucosidase キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Saliva, Serum, Cell culture extracts, Tissue, Adherent cells, Suspension cells -
アッセイタイプ
Enzyme activity (quantitative) -
検出範囲
0.1 mU/well - 10 mU/well -
種交差性
交差種: Mammals, Other species -
製品の概要
In Abcam's Alpha-Glucosidase Activity Assay Kit (Colorimetric) (ab174093), α-Glucosidase hydrolyzes the Substrate Mix to release the p-nitrophenol that can be measured colorimetrically (OD = 410 nm). This is an easy, quick and high-throughput capable kit that can measure 0.1-10 mU of α-glucosidase activity in a variety of samples.
Visit our FAQs page for tips and troubleshooting.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K690 α-Glucosidase Activity Colorimetric Assay Kit. K690-100 is the same size as the 100 test size of ab174093.
α-Glucosidase breaks down α-1,4 linked polysaccharides to glucose, which can be utilized as a source of energy. In the biotechnology industry, α-glucosidase is used to produce glucose from intermediate breakdown products of starch hydrolysis generated by enzymes such as amylase.
Pompe disease, one of the 12 known glycogen storage diseases, is an autosomal recessive metabolic disorder attributed to α-glucosidase deficiency. In this disease, glycogen accumulates in the lysosomes, resulting in progressive muscle weakness, heart failure and other neurological symptoms.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests α-Glucosidase Assay Buffer 1 x 25ml α-Glucosidase Positive Control 1 vial α-Glucosidase Substrate Mix 1 x 0.3ml p-Nitrophenol Standard 1 x 100µl -
研究分野
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関連性
a-Glucosidase breaks down a-1,4 linked polysaccharides to glucose, which can be utilized as a source of energy. In the biotechnology industry, a-glucosidase is used to produce glucose from intermediate breakdown products of starch hydrolysis generated by enzymes such as amylase. Pompe disease, one of the 12 known glycogen storage diseases, is an autosomal recessive metabolic disorder attributed to a- glucosidase deficiency. In this disease, glycogen accumulates in the lysosomes, resulting in progressive muscle weakness, heart failure and other neurological symptoms. -
細胞内局在
Lysosome. Lysosome membrane. -
別名
- Acid Maltase
- Aglucosidase Alfa
- EC 3.2.1.20
see all
画像
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Time course of Alpha-Glucosidase Activity in mouse liver tissue lysate
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Time course of Alpha-Glucosidase Activity in various samples
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Kinetic profile of various amounts (0, 2, 4, 6, 8 & 10 mU) of α-glucosidase run at 25°C under this protocol. Inset: Results for 0-0.2-0.4-0.6-0.8-1.0 mU of α-glucosidase. Data points after 5 minutes were used to determine slope. This is example data only.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab174093 は 2 報の論文で使用されています。
- Yang H et al. Effects of iron oxide nanoparticles as T2-MRI contrast agents on reproductive system in male mice. J Nanobiotechnology 20:98 (2022). PubMed: 35236363
- Khamoui AV et al. Hepatic proteome analysis reveals altered mitochondrial metabolism and suppressed acyl-CoA synthetase-1 in colon-26 tumor-induced cachexia. Physiol Genomics 52:203-216 (2020). PubMed: 32146873