Anti-alpha COP I/COPA 抗体 (ab2913)
Key features and details
- Rabbit polyclonal to alpha COP I/COPA
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-alpha COP I/COPA antibody
alpha COP I/COPA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to alpha COP I/COPA -
由来種
Rabbit -
特異性
Detects coatomer-protein I alpha (COP I alpha). -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow, Dog, Rice -
免疫原
Synthetic peptide corresponding to Rat alpha COP I/COPA aa 1-19.
Sequence:MLTKFETKSARVKGLSFHP
(Peptide available asab4931) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
一次抗体 備考
Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2913の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 3 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 3 µg/ml. |
ターゲット情報
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機能
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor. -
組織特異性
Uniformly expressed in a wide range of adult and fetal tissues. Xenin is found in gastric, duodenal and jejunal mucosa. Circulates in the blood. Seems to be confined to specific endocrine cells. -
配列類似性
Contains 6 WD repeats. -
発生段階
Xenin is released into the circulation after a meal. -
細胞内局在
Secreted and Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. - Information by UniProt
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参照データベース
- Entrez Gene: 100126041 Cow
- Entrez Gene: 1314 Human
- Entrez Gene: 12847 Mouse
- Omim: 601924 Human
- SwissProt: Q27954 Cow
- SwissProt: P53621 Human
- SwissProt: Q8CIE6 Mouse
- Unigene: 162121 Human
see all -
別名
- Alpha coat protein antibody
- Alpha COP antibody
- Alpha COPI antibody
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab2913 は 1 報の論文で使用されています。
- Sudo H et al. Knockdown of COPA, identified by loss-of-function screen, induces apoptosis and suppresses tumor growth in mesothelioma mouse model. Genomics 95:210-6 (2010). WB ; Human . PubMed: 20153416