Anti-alpha COP I/COPA 抗体 (ab2913)

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Key features and details

  • Rabbit polyclonal to alpha COP I/COPA
  • Suitable for: ICC/IF
  • Reacts with: Rat, Human, Non human primates
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-alpha COP I/COPA antibody
    alpha COP I/COPA 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to alpha COP I/COPA

  • 由来種

    Rabbit

  • 特異性

    Detects coatomer-protein I alpha (COP I alpha).

  • アプリケーション

    適用あり: ICC/IFmore details

  • 種交差性

    交差種: Rat, Human, Non human primates
    交差が予測される動物種: Mouse, Cow, Dog, Rice

  • 免疫原

    Synthetic peptide corresponding to Rat alpha COP I/COPA aa 1-19.
    Sequence:

    MLTKFETKSARVKGLSFHP


    (Peptide available as ab4931)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

     This product was previously labelled as alpha COP I

     

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 一次抗体 備考

    Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 3 µg/ml.

ターゲット情報

  • 機能

    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
    Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor.

  • 組織特異性

    Uniformly expressed in a wide range of adult and fetal tissues. Xenin is found in gastric, duodenal and jejunal mucosa. Circulates in the blood. Seems to be confined to specific endocrine cells.

  • 配列類似性

    Contains 6 WD repeats.

  • 発生段階

    Xenin is released into the circulation after a meal.

  • 細胞内局在

    Secreted and Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Alpha coat protein antibody
    • Alpha COP antibody
    • Alpha COPI antibody
    • Alpha-coat protein antibody
    • Alpha-COP antibody
    • AlphaCOP antibody
    • Coatomer protein complex subunit alpha antibody
    • Coatomer subunit alpha antibody
    • COP A antibody
    • COP I alpha antibody
    • copA antibody
    • COPA_HUMAN antibody
    • COPI alpha antibody
    • FLJ26320 antibody
    • HEP COP antibody
    • HEP-COP antibody
    • HEPCOP antibody
    • Proxenin antibody
    • Xenin antibody
    • Xenopsin-related peptide antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha COP I/COPA antibody (ab2913)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha COP I/COPA antibody (ab2913)

    ICC/IF of COP1 in Hela Cells

参考文献 (1)

ab2913 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2913 は 1 報の論文で使用されています。

  • Sudo H  et al. Knockdown of COPA, identified by loss-of-function screen, induces apoptosis and suppresses tumor growth in mesothelioma mouse model. Genomics 95:210-6 (2010). WB ; Human . PubMed: 20153416

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Question

Here's the detailed explanation: we bought 2 antibody from you company , alpha-COP and delta-COP, these two protein are both subunits of COPI vesicle and they both in out layer of COPI , so it means these two proteins should localized at pretty similar position in cells, both outside nuclei, golgi-like morphology. we used these two antibodies for confocal experiment on Hela cells, we got quite different result for these two antibody, the delta-COP one work well, which has right morphology while the alpha-COP showed wield localization in the cell, which is inside nuclei. I attached the confocal data (Image) and protocol . based on the known knowledge, there is something wrong with the alpha-COP antibody, please help us to solve this problem. Thank you very much for your help,

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Answer

Thank you for the explanation, and for the image and protocol. The staining you are seeing with the alpha COP anditboy ab2913 does look incorrect, if all the staining is expected to be peri-nuclear, as is seen with the delta COP antibody. The small black and white image of HeLa cells on the ab2913 datasheet also shows what appears to be intra-nuclear staining, along with the ER/Golgi stain. This suggests that the antibody routinely cross-reacts with a nuclear protein that is not alpha COP (unless the apparent nuclear stain is actually COP on top of the nuclei). We have not received other complaints, but few people have tried ab2913. I suggest replacing the antibody with another, but the only other alpha COP antibody in our catalogue is ab104710, and this has only been tested for western blotting. It may work for ICC, but you will need to optimize the stain. If you are interested in trying this, I will send a vial free-of-charge, to replace ab2913. Click here (or use the following: https://www.abcam.com/index.html?datasheet=104710). If you prefer, I can send an antibody against a different target, for instance the beta COP antibody ab2899: Click here (or use the following: https://www.abcam.com/index.html?datasheet=2899). I can also issue a credit or refund, if you prefer. I look forward to your reply.

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Question

Hello Abcam,   We ordered a rabbit polyclonal to alpha COP1, but it wasn't performing as promised; the morphology was wrong. Please send us forms for a replacement/refund, and we will provide you with all the data.

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Answer

Thank you for contacting us. I am sorry to read that you are having trouble getting a good result with this antibody. Can you please tell me what application you are using it for? I am assuming IHC or ICC but I will need a few more details. We always ask for sample and protocol information in these instances, in the hope that we can make a suggestion that improves the result. If the application and species are covered by our Abpromise guarantee, and the antibody was purchased within the past 6 months, we can send a replacement or refund if we we are unable to suggest a solution for the problem.

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Question

I am interested in your product,alpha COP antibody (ab2913, and I have question about this. Do you have any Western Blotting data tested to the antibody? What I want to know is that the antibody detect unknown protein, or the alpha cop antibody detect only for alpha COP. I read the datasheet on the web-site. I learn your product is a polyclonal antibody. If the antibody could detect,which size potein and how many kinds of unknowm (or non-specific)the antibody detect on the Western Bottling. I am expectin your reply. Thank you

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Answer

Thank you for your email. Ab2913 was tested for application in Western blotting using PC-12 cell extract. The antibody detected a band at approximately 140 kDa representing COPI alpha. The antibody also detected a band at approximately 100 kDa, the identity of which is unknown. We currently do not have an image, but we do guarantee that the antibody will work in Western blotting and cross-react with human and rat alpha COP I. I would recommend using PC-12 cell extract as a positive control and we also sell the blocking peptide (ab4931) which can be used in control experiments. Please contact us again if you have any additional questions.

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Question

I'm so sorry but I miswrote the application what our customer used. I wrote that they demonstrated the 'Immunocytochemistry' using #AB2913, Indeed, they demonstrated the 'Immunocytometry' by using this antibody, not 'Immunohistchemistry' I describe the detail of their experimental condition as follows. They demonstrated 'Immunocytometry' using #AB2913. The Lot# was 51911 In their experiment, they could not detect any fluorescence signal. They stained the HeLa cell and did not fix the samples. The ratio of the dilution of primary antibody was 1:300. Reaction was performed at 37 degree centigrade for 90 minutes. The anti-goat IgG antibody (raised in Rabbit) which conjugated with Alexa Fluor 488 (Molecular Probe Inc.) was used for the secondary antibody. The ratio of the dilution of secondary antibody was 1:100. They already checked the secondary antibody worked well using anti- AP-2 and beta-COP antibodies as the primary antibody . Positive and negative control was not used.

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Answer

Thank you for submitting the enquiry. The customer is using an inappropriate secondary Ab. The primary Ab is raised in rabbit and so an anti rabbit secondary is necessary not anti goat! If you have any further questions then please do not hesitate to get back in touch with me.

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