Anti-Alpha B Crystallin 抗体 (ab13497)
Key features and details
- Rabbit polyclonal to Alpha B Crystallin
- Suitable for: IP, WB, ICC, IHC-Fr, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Chicken, Cow, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-Alpha B Crystallin antibody
Alpha B Crystallin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Alpha B Crystallin -
由来種
Rabbit -
特異性
Does not cross-react with αA-crystallin.
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アプリケーション
適用あり: IP, WB, ICC, IHC-Fr, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Chicken, Cow, Human, Pig
交差が予測される動物種: Rabbit, Hamster -
免疫原
Synthetic peptide:
REEKPAVTAAPKK
conjugated to KLH, corresponding to amino acids 163-175 of Human alpha B Crystallin. -
ポジティブ・コントロール
- ICC: HeLa. (Heat shocked). WB: A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 , and Rat brain cell lysate.
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特記事項
For maximum product recovery, after thawing, centrifuge the product vial before removing capThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13497の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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WB | (3) |
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).
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ICC |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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ICC/IF |
1/120.
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特記事項 |
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IP
Use at an assay dependent concentration. |
WB
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa). |
ICC
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/120. |
ターゲット情報
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機能
May contribute to the transparency and refractive index of the lens. -
組織特異性
Lens as well as other tissues. -
関連疾患
Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients. -
配列類似性
Belongs to the small heat shock protein (HSP20) family. -
細胞内局在
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. - Information by UniProt
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参照データベース
- Entrez Gene: 396089 Chicken
- Entrez Gene: 281719 Cow
- Entrez Gene: 1410 Human
- Entrez Gene: 12955 Mouse
- Entrez Gene: 100127443 Pig
- Entrez Gene: 100009295 Rabbit
- Entrez Gene: 25420 Rat
- Omim: 123590 Human
see all -
別名
- AACRYA antibody
- Alpha B crystallin antibody
- Alpha crystallin B chain antibody
see all
画像
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Anti-Alpha B Crystallin antibody (ab13497) at 1/1000 dilution + Human spinal cord tissue lysate - total protein (ab29188) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 20 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted? -
Western blot analysis of Human A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 and T98G cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ab13497).
Lane 1: Molecular Weight Ladder (MW).
Lane 2: A431 cell lysates.
Lane 3: HCT116 cell lysates.
Lane 4: HeLa cell lysates.
Lane 5: HepG2 cell lysates.
Lane 6: HEK293 cell lysates.
Lane 7: HUVEC cell lysates.
Lane 8: Jurkat cell lysates.
Lane 9: MCF7 cell lysates.
Lane 10: PC3 cell lysates.
Lane 11: T98G cell lysates.
Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ab13497) at 1/1000 dilution for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1/1000 dilution for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.
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Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ab13497).
Lane 1: Molecular Weight Ladder (MW).
Lane 2: Rat Brain cell lysates.
Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ab13497) at 1/1000 dilution for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1/1000 dilution for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.
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Immunofluorescent analysis of 2% paraformaldehyde fixed Heat Shocked (at 42°C for 1h) Cervical cancer cell line (HeLa) for 20min at RT, labelling Alpha B Crystallin with ab13497 at 1/120 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (FITC) (Green) at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at RT. Image showing actin filament bundles, nuclear splicing speckles, and Exosome staining. (A) DAPI (blue) nuclear stain. (B) Anti-Alpha B Crystallin Antibody (green). (C) Composite image.
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Immunofluorescent analysis of 2% paraformaldehyde fixed Heat Shocked (at 42°C for 1h) Cervical cancer cell line (HeLa) for 20min at RT, labelling Alpha B Crystallin with ab13497 at 1/120 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (APC) (Red) at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at RT. Image showing actin filament bundles, nuclear splicing speckles, and Exosome staining. (A) DAPI (blue) nuclear stain. (B) Anti-Alpha B Crystallin Antibody (Red). (C) Composite image.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (19)
ab13497 は 19 報の論文で使用されています。
- Dumas SJ et al. Single-Cell RNA Sequencing Reveals Renal Endothelium Heterogeneity and Metabolic Adaptation to Water Deprivation. J Am Soc Nephrol 31:118-138 (2020). PubMed: 31818909
- Besnard V et al. Identification of periplakin as a major regulator of lung injury and repair in mice. JCI Insight 3:N/A (2018). PubMed: 29515024
- Zhang Y et al. BYD Ameliorates Oxidative Stress-Induced Myocardial Apoptosis in Heart Failure Post-Acute Myocardial Infarction via the P38 MAPK-CRYAB Signaling Pathway. Front Physiol 9:505 (2018). PubMed: 29867551
- Schmitt A et al. Effects of 10 weeks of regular running exercise with and without parallel PDTC treatment on expression of genes encoding sarcomere-associated proteins in murine skeletal muscle. Cell Stress Chaperones 23:1041-1054 (2018). PubMed: 29797237
- Guo H et al. Exercise-responsive phosphoproteins in the heart. J Mol Cell Cardiol 111:61-68 (2017). WB . PubMed: 28826663