Anti-Alpha B Crystallin 抗体 [1B6.1-3G4] (ab13496)
Key features and details
- Mouse monoclonal [1B6.1-3G4] to Alpha B Crystallin
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Cow, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Alpha B Crystallin antibody [1B6.1-3G4]
Alpha B Crystallin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1B6.1-3G4] to Alpha B Crystallin -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cyt, IHC-Pmore details -
種交差性
交差種: Cow, Human
交差が予測される動物種: Mouse, Rat, Chicken -
免疫原
Full length native protein (purified) corresponding to Cow Alpha B Crystallin.
Database link: P02510 -
ポジティブ・コントロール
- Recombinant Bovine alpha B Crystallin Protein.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 49.91% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
1B6.1-3G4 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13496の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P | (1) |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa). |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
May contribute to the transparency and refractive index of the lens. -
組織特異性
Lens as well as other tissues. -
関連疾患
Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients. -
配列類似性
Belongs to the small heat shock protein (HSP20) family. -
細胞内局在
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. - Information by UniProt
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参照データベース
- Entrez Gene: 396089 Chicken
- Entrez Gene: 281719 Cow
- Entrez Gene: 1410 Human
- Entrez Gene: 12955 Mouse
- Entrez Gene: 25420 Rat
- Omim: 123590 Human
- SwissProt: Q05713 Chicken
- SwissProt: P02510 Cow
see all -
別名
- AACRYA antibody
- Alpha B crystallin antibody
- Alpha crystallin B chain antibody
see all
画像
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Overlay histogram showing SH-SY5Y cells stained with ab13496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13496, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
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Immunohistochemistry (paraffin sections) analysis of Human heart tissue labelling Alpha B Crystallin with ab13496 at 20 µg/mL
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All lanes : Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496) at 1/1000 dilution
Lane 2 : a-Crystallin (bovine), (native)
Lane 3 : aB-Crystallin (bovine), (native)
Predicted band size: 21 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (55)
ab13496 は 55 報の論文で使用されています。
- Falcone C et al. Redefining varicose projection astrocytes in primates. Glia 70:145-154 (2022). PubMed: 34533866
- Lee B et al. Associations of Apoptotic and Anti-Apoptotic Factors with Beef Quality, Histochemical Characteristics, and Palatability of Hanwoo Longissimus thoracis Muscle. Animals (Basel) 12:N/A (2022). PubMed: 35203175
- Quinlan PR et al. Cluster analyses of the TCGA and a TMA dataset using the coexpression of HSP27 and CRYAB improves alignment with clinical-pathological parameters of breast cancer and suggests different epichaperome influences for each sHSP. Cell Stress Chaperones 27:177-188 (2021). PubMed: 35235182
- Della Marina A et al. Clinical Course, Myopathology and Challenge of Therapeutic Intervention in Pediatric Patients with Autoimmune-Mediated Necrotizing Myopathy. Children (Basel) 8:N/A (2021). PubMed: 34572153
- Falcone C et al. Cortical Interlaminar Astrocytes Are Generated Prenatally, Mature Postnatally, and Express Unique Markers in Human and Nonhuman Primates. Cereb Cortex 31:379-395 (2021). PubMed: 32930323