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Anti-Alpha B Crystallin 抗体 [1B6.1-3G4] (ab13496)

Reviews (5)Q&A (3)References (44)
Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
  • Flow Cytometry - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
  • Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)

Key features and details

  • Mouse monoclonal [1B6.1-3G4] to Alpha B Crystallin
  • Suitable for: WB, ICC, IP, IHC-Fr, IHC-FrFl, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Chicken, Cow, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Alpha B Crystallin antibody [1B6.1-3G4]
    Alpha B Crystallin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [1B6.1-3G4] to Alpha B Crystallin

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, ICC, IP, IHC-Fr, IHC-FrFl, Flow Cyt, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Chicken, Cow, Human

  • 免疫原

    Full length native protein (purified) corresponding to Cow Alpha B Crystallin.

  • ポジティブ･コントロール

    • Recombinant Bovine alpha B Crystallin Protein.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 31st May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab13496の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).
ICC
Use at an assay dependent concentration.
IP (1)
Use at an assay dependent concentration.
IHC-Fr (1)
Use at an assay dependent concentration.
IHC-FrFl (1)
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 
IHC-P (1)
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).
ICC
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-FrFl
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 
IHC-P
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    May contribute to the transparency and refractive index of the lens.

  • 組織特異性

    Lens as well as other tissues.

  • 関連疾患

    Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.

  • 配列類似性

    Belongs to the small heat shock protein (HSP20) family.

  • 細胞内局在

    Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AACRYA antibody
    • Alpha B crystallin antibody
    • Alpha crystallin B chain antibody
    • Alpha(B)-crystallin antibody
    • Alpha-crystallin B chain antibody
    • CRYA2 antibody
    • Cryab antibody
    • CRYAB_HUMAN antibody
    • Crystallin alpha B antibody
    • Crystallin alpha polypeptide 2 antibody
    • CTPP2 antibody
    • Heat shock 20 kD like protein antibody
    • Heat shock protein beta 5 antibody
    • Heat shock protein beta-5 antibody
    • HspB5 antibody
    • Renal carcinoma antigen NY REN 27 antibody
    • Renal carcinoma antigen NY-REN-27 antibody
    • Rosenthal fiber component antibody
    see all

画像

  • Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    All lanes : Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : alpha Crystallin

    Lane 3 : alpha A Crystallin

    Lane 4 : alpha B Crystallin
    Lane 5 : beta Crystallin

    Predicted band size: 21 kDa



    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)

    Immunohistochemical analysis of human heart tissue using ab13496 at the concentration 10ug/ml.

    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    Flow Cytometry - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)

    Overlay histogram showing SH-SY5Y cells stained with ab13496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13496, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)
    Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)

    This image was generated using the ascites version of the product.

参考文献 (44)

ab13496 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab13496 は 44 報の論文で使用されています。

  • Liu Y  et al. Peripherally misfolded proteins exacerbate ischemic stroke-induced neuroinflammation and brain injury. J Neuroinflammation 18:29 (2021). PubMed: 33472658
  • Ou MY  et al. The CTNNBIP1-CLSTN1 fusion transcript regulates human neocortical development. Cell Rep 35:109290 (2021). PubMed: 34192541
  • Yang L  et al. Developmental Origins of Human Cortical Oligodendrocytes and Astrocytes. Neurosci Bull N/A:N/A (2021). PubMed: 34374948
  • Baburamani AA  et al. Assessment of radial glia in the frontal lobe of fetuses with Down syndrome. Acta Neuropathol Commun 8:141 (2020). PubMed: 32819430
  • de la Fuente AG  et al. Changes in the Oligodendrocyte Progenitor Cell Proteome with Ageing. Mol Cell Proteomics 19:1281-1302 (2020). PubMed: 32434922
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-8 of 8 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Alpha B Crystallin antibody [1B6.1-3G4]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric Acid Buffer, pH 6 (with 0.1% Tween20) Steamer
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Read More

Dr. Ana Baburamani

Verified customer

投稿 Nov 16 2020

Immunohistochemistry (Frozen sections) abreview for Anti-Alpha B Crystallin antibody [1B6.1-3G4]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample
Rat Tissue sections (brain, 20 micron-thick free-floating sections)
Specification
brain, 20 micron-thick free-floating sections
Permeabilization
No
Fixative
Paraformaldehyde
Read More

DR. Marie-Caroline Cotel

Verified customer

投稿 May 28 2014

Immunoprecipitation abreview for Anti-Alpha B Crystallin antibody [1B6.1-3G4]

 Excellent
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (muscle)
Total protein in input
20 µg
Specification
muscle
Immuno-precipitation step
Protein G
Read More

Abcam user community

Verified customer

投稿 Apr 19 2012

Western blot abreview for Anti-Alpha B Crystallin antibody [1B6.1-3G4]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole brain homogenate)
Loading amount
10 µg
Specification
Whole brain homogenate
Gel Running Conditions
Reduced Denaturing (Bis-tris 4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Mr. Brian Hitt

Verified customer

投稿 May 07 2010

Immunohistochemistry - Free Floating abreview for Anti-Alpha B Crystallin antibody [1B6.1-3G4]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Read More

Mr. Brian Hitt

Verified customer

投稿 Apr 30 2010

Question

I would like to try the anti-alpha B Crystallin antibody (ab13496) for immunoprecipitation on mouse striated muscle.  

Read More
Answer

DISCOUNT CODE: *** Expiration date: April 19th, 2012 I am very pleased to hear you would like to accept our offer and test ab13496 in IP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question

Would like to try a VEGFC antibody. High background in WB with ab83339 and ab13496.

Read More
Answer

Thanks for your call today and for letting us know about the trouble with ab83339 and ab13496. Please keep me updated about any progress with these antibodies, and let me know if the protocol alterations we discussed are not helpful. Also, we have a few VEGFC antibodies that have been tested in WB with human samples, but ab9546 is our most frequently ordered and has been used in 6 publications- https://www.abcam.com/VEGFC-antibody-ab9546.html Please let me know if you would like to try this antibody or a different one and I can issue a discount code for you. I look forward to hearing from you. If you have any questions don't hesitate to ask! Have a great day.  

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Question

When this antibody was tested in IHC, was it tested with formalin fixed paraffin embedded sections, frozen sections, or both?

Read More
Answer

Thank you for your enquiry. Ab13496 has been tested for application in IHC-frozen sections and has not yet been tested in IHC-formalin fixed paraffin embedded sections. If you have any additional questions, please contact us again.

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