Application
Immunocytochemistry/ Immunofluorescence
Blocking step
0.1% BSA and 1% serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Sample
Mouse Cell (mouse embryonic stem cell)
Specification
mouse embryonic stem cell
Permeabilization
Yes - 0.1% triton x
Fixative
methanol/acetone
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 16°C · Diluent: blocking buffer
Dilution
1/200
Secondary antibody
Name
Abcam antibody:Goat Anti-Mouse IgG H&L (Alexa Fluor 488) preadsorbed
Dilution
1/500
Additional data
Additional Notes
Wt mES cells stained with alpha-Adaptin antibody. A mutant cell line with reduced alpha-Adaptin expression was also tested and reduced staining was seen. PFA fixation was also tested, but staining was very diffuse and non-specific.
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投稿 May 19 2014