Name: Anti-alpha Adaptin antibody [AP6]
SKU: ab2730
Rating: 4 (10 reviews)

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Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)

Key features and details

Mouse monoclonal [AP6] to alpha Adaptin
Suitable for: ICC/IF, Flow Cyt
Reacts with: Mouse, Human
Isotype: IgG1

製品名

Anti-alpha Adaptin antibody [AP6]
alpha Adaptin 一次抗体製品一覧
製品の詳細

Mouse monoclonal [AP6] to alpha Adaptin
由来種

Mouse
特異性

Detects assembly polypeptide 2 (AP2) It recognizes the products of both alpha-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha A found in neurons.
アプリケーション

適用あり: ICC/IF, Flow Cytmore details
種交差性

交差種: Mouse, Human
交差が予測される動物種: Non human primates
免疫原

Full length native protein (purified) corresponding to alpha Adaptin. Purified adaptors.
特記事項

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファー

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
精製度

Affinity purified
ポリ/モノ

モノクローナル
クローン名

AP6
アイソタイプ

IgG1
研究分野

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab2730の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
ICC/IF	(4)	Use a concentration of 5 µg/ml.
Flow Cyt		Use 1µg for 10⁶ cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

特記事項
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 10⁶ cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

関連性

Clathrin mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down regulated. During formation of clathrin coated membranes, clathrin co assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.
細胞内局在

Cytoplasmic
参照データベース
- Entrez Gene: 160 Human
- Entrez Gene: 161 Human
- Entrez Gene: 11771 Mouse
- Entrez Gene: 11772 Mouse
- Omim: 601026 Human
- Omim: 607242 Human
- SwissProt: O94973 Human
- SwissProt: O95782 Human
- SwissProt: P17426 Mouse
- SwissProt: P17427 Mouse
see all
別名
- 100 kDa coated vesicle protein A antibody
- 100 kDa coated vesicle protein C antibody
- Adapter related protein complex 2 subunit alpha 1 antibody
- Adapter related protein complex 2 subunit alpha 2 antibody
- Adaptin alpha A antibody
- Adaptin alpha B antibody
- Adaptin, alpha B antibody
- Adaptor protein complex AP 2 alpha 1 subunit antibody
- Adaptor protein complex AP 2 alpha 2 subunit antibody
- Adaptor protein complex AP 2 subunit alpha 1 antibody
- Adaptor related protein complex 2 alpha 1 subunit antibody
- Adaptor related protein complex 2 alpha 2 subunit antibody
- ADTAA antibody
- ADTAB antibody
- Alpha adaptin A antibody
- Alpha adaptin C antibody
- Alpha adaptin C; Huntingtin interacting protein J antibody
- Alpha1 adaptin antibody
- Alpha2 adaptin antibody
- AP 2 complex subunit alpha 1 antibody
- AP 2 complex subunit alpha 2 antibody
- AP2 alpha antibody
- AP2A1 antibody
- AP2A2 antibody
- CLAPA1 antibody
- CLAPA2 antibody
- Clathrin assembly protein complex 2 alpha A large chain antibody
- Clathrin assembly protein complex 2 alpha C large chain antibody
- Clathrin associated/assembly/adaptor protein large alpha 1 antibody
- Clathrin associated/assembly/adaptor protein large alpha 2 antibody
- HIP 9 antibody
- HIP9 antibody
- Huntingtin interacting protein antibody
- Huntingtin interacting protein HYPJ antibody
- Huntingtin interacting protein J antibody
- Huntingtin yeast partner J antibody
- HYPJ antibody
- Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody
- Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)

Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2730) shows staining in U251 Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2730) at a dilution of 1:20 over night at 4C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)

Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2720) shows staining in MCF-7 Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2730) at a dilution of 1:20 over night at 4 C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)

Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2730) shows staining in Hela Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2720) at a dilution of 1:20 over night at 4 C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)

ICC/IF image of ab2730 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2730, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry - Anti-alpha Adaptin antibody [AP6] (ab2730)

Overlay histogram showing MCF7 cells stained with ab2730 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2730, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

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Datasheet download

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ab2730 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2730 は 21 報の論文で使用されています。

Wagner W et al. Myosin VI Drives Clathrin-Mediated AMPA Receptor Endocytosis to Facilitate Cerebellar Long-Term Depression. Cell Rep 28:11-20.e9 (2019). PubMed: 31269433
Charnley M et al. A new role for Notch in the control of polarity and asymmetric cell division of developing T cells. J Cell Sci 133:N/A (2019). PubMed: 31578237
Evergren E et al. Eps15R and clathrin regulate EphB2-mediated cell repulsion. Traffic 19:44-57 (2018). PubMed: 28972287
Hugo SE & Schlegel A A Genetic Model to Study Increased Hexosamine Biosynthetic Flux. Endocrinology 158:2420-2426 (2017). PubMed: 28582574
Humphries AC et al. Cdc42 and the Rho GEF intersectin-1 collaborate with Nck to promote N-WASP-dependent actin polymerisation. J Cell Sci 127:673-85 (2014). PubMed: 24284073

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-10 of 14 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (undifferentiated Human ES cells on MEFs)

Permeabilization

Yes - in blocking buffer

Specification

undifferentiated Human ES cells on MEFs

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 Feb 01 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (Undifferentiated Human ES cells on MEFs)

Gel Running Conditions

Non-reduced Denaturing (12.5%)

Loading amount

20 µg

Specification

Undifferentiated Human ES cells on MEFs

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

投稿 Jan 25 2016

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HEPG2)

Specification

HEPG2

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 Nov 18 2015

Application

Western blot

Sample

Human Cell lysate - whole cell (HEPG2)

Gel Running Conditions

Reduced Denaturing (12.5)

Loading amount

20 µg

Specification

HEPG2

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

Abcam user community

Verified customer

投稿 Nov 13 2015

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

0.1% BSA and 1% serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C

Sample

Mouse Cell (mouse embryonic stem cell)

Specification

mouse embryonic stem cell

Permeabilization

Yes - 0.1% triton x

Fixative

methanol/acetone

Abcam user community

Verified customer

投稿 May 19 2014

Application

Western blot

Loading amount

100000 cells

Gel Running Conditions

Reduced Denaturing (10% gel)

Sample

Mouse Cell lysate - whole cell (mouse embryonic stem cells)

Specification

mouse embryonic stem cells

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

Abcam user community

Verified customer

投稿 Jan 09 2014

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C

Sample

Human Cell (HeLa)

Specification

HeLa

Permeabilization

Yes - 0.2% Triton in PBS

Fixative

Formaldehyde

Abcam user community

Verified customer

投稿 Mar 05 2013

Application

Western blot

Sample

Mouse Cell lysate - whole cell (mouse neuro2A)

Loading amount

90 µg

Specification

mouse neuro2A

Gel Running Conditions

Non-reduced Non-Denaturing (Native) (8% running gel)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Dec 21 2012

Question

Thank you for your reply. Since we got AP2 antibody from sigma and that antibody works, we did not really do trouble-shooting. About the refund, we always buy antibody from your company, so I am thinking that the active code that can be used at next purchase for the same price products may be good for us. Thank you.Best

Abcam community

Verified customer

Asked on Dec 21 2012

Answer

Thank you for contacting us.

Your credit note ID is XXXXX.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Abcam Scientific Support

Answered on Dec 21 2012

Question

ReviewID: 33690 AbID: 2730, Anti-alpha Adaptin antibody [AP6] Rating: Inconclusive Image: Anti-alpha Adaptin antibody [AP6] for Western blot (Mouse) Sample: Species: Mouse Type: Cell lysate - whole cell Loading amount: 90 µg Specification: mouse neuro2A Application: Application: Gel Running Conditions: Non-reduced Non-Denaturing (Native) (8% running gel) Blocking step: Milk as blocking agent for 1 hour at 25°C Blocking Concentration: 5% Other detail: Dilution: 1/300 Incubation time: 12 hours at 4°C Secondary Antibody: Name: Non-Abcam Antibody was used: anti-mouse IgG(whole molecule)-peroxidase, antibod Conjugation: HRP Dilution: 1/5000 Detection: Detection method: ECL Exposure: 30 minutes and 0 seconds Bands: Specific: no kDa Non-specific: too many kDa Positive Control: we use sigma A4325 monoclonal anti-a-adaptin (AP2)as a control,same sample solubilization, it is able to detecting AP2 pretty well. Additional Data:

Abcam community

Verified customer

Asked on Dec 21 2012

Answer

Thank you for submitting an Abreview of ab2730. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

Based on the information in your review, I feel that altering few step in your protocol may help improve your results. I wonder if you have tried troubleshooting the protocol. If yes, could you provide details.

This antibody is covered by our Abpromise, so if the antibody was purchased within the past 6 months, I would be happy to discuss a refund or replacement with you to resolve this issue.

I look forward to your reply.

Abcam Scientific Support

Answered on Dec 21 2012

1-10 of 14 Abreviews or Q&A

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Key features and details

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Compatible Secondaries

Conjugation kits

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

関連性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (21)

レビューと Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Adaptin antibody [AP6]

Western blot abreview for Anti-alpha Adaptin antibody [AP6]

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Adaptin antibody [AP6]

Western blot abreview for Anti-alpha Adaptin antibody [AP6]

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Adaptin [AP6] antibody

Western blot abreview for Anti-alpha Adaptin antibody [AP6]

Immunocytochemistry/ Immunofluorescence

Western blot abreview for Anti-alpha Adaptin antibody [AP6]