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Anti-alpha Adaptin 抗体 [AP6] (ab2730)

  • Datasheet
  • SDS
Reviews (10)Q&A (4)References (21)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
  • Flow Cytometry - Anti-alpha Adaptin antibody [AP6] (ab2730)

Key features and details

  • Mouse monoclonal [AP6] to alpha Adaptin
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Hamster, Cow, Human
  • Isotype: IgG1

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関連製品

製品の概要

  • 製品名

    Anti-alpha Adaptin antibody [AP6]
    alpha Adaptin 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [AP6] to alpha Adaptin
  • 由来種

    Mouse
  • 特異性

    Detects assembly polypeptide 2 (AP2) It recognizes the products of both alpha-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha A found in neurons.
  • アプリケーション

    適用あり: ICC/IF, Flow Cytmore details
  • 種交差性

    交差種: Mouse, Rat, Hamster, Cow, Human
    交差が予測される動物種: Non human primates
  • 免疫原

    Other Immunogen Type corresponding to alpha Adaptin. Purified adaptors.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Affinity purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    AP6
  • アイソタイプ

    IgG1
  • 研究分野

    • Signal Transduction
    • Adapters
    • Transmembrane

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

アプリケーション

Our Abpromise guarantee covers the use of ab2730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 関連性

    Clathrin mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down regulated. During formation of clathrin coated membranes, clathrin co assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.
  • 細胞内局在

    Cytoplasmic
  • 参照データベース

    • Entrez Gene: 515216 Cow
    • Entrez Gene: 784636 Cow
    • Entrez Gene: 160 Human
    • Entrez Gene: 161 Human
    • Entrez Gene: 11771 Mouse
    • Entrez Gene: 11772 Mouse
    • Entrez Gene: 308578 Rat
    • Entrez Gene: 81637 Rat
    • Omim: 601026 Human
    • Omim: 607242 Human
    • SwissProt: Q0VCK5 Cow
    • SwissProt: O94973 Human
    • SwissProt: O95782 Human
    • SwissProt: P17426 Mouse
    • SwissProt: P17427 Mouse
    • SwissProt: P18484 Rat
    • Unigene: 19121 Human
    • Unigene: 6877 Mouse
    • Unigene: 34928 Rat
    see all
  • 別名

    • 100 kDa coated vesicle protein A antibody
    • 100 kDa coated vesicle protein C antibody
    • Adapter related protein complex 2 subunit alpha 1 antibody
    • Adapter related protein complex 2 subunit alpha 2 antibody
    • Adaptin alpha A antibody
    • Adaptin alpha B antibody
    • Adaptin, alpha B antibody
    • Adaptor protein complex AP 2 alpha 1 subunit antibody
    • Adaptor protein complex AP 2 alpha 2 subunit antibody
    • Adaptor protein complex AP 2 subunit alpha 1 antibody
    • Adaptor related protein complex 2 alpha 1 subunit antibody
    • Adaptor related protein complex 2 alpha 2 subunit antibody
    • ADTAA antibody
    • ADTAB antibody
    • Alpha adaptin A antibody
    • Alpha adaptin C antibody
    • Alpha adaptin C; Huntingtin interacting protein J antibody
    • Alpha1 adaptin antibody
    • Alpha2 adaptin antibody
    • AP 2 complex subunit alpha 1 antibody
    • AP 2 complex subunit alpha 2 antibody
    • AP2 alpha antibody
    • AP2A1 antibody
    • AP2A2 antibody
    • CLAPA1 antibody
    • CLAPA2 antibody
    • Clathrin assembly protein complex 2 alpha A large chain antibody
    • Clathrin assembly protein complex 2 alpha C large chain antibody
    • Clathrin associated/assembly/adaptor protein large alpha 1 antibody
    • Clathrin associated/assembly/adaptor protein large alpha 2 antibody
    • HIP 9 antibody
    • HIP9 antibody
    • Huntingtin interacting protein antibody
    • Huntingtin interacting protein HYPJ antibody
    • Huntingtin interacting protein J antibody
    • Huntingtin yeast partner J antibody
    • HYPJ antibody
    • Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody
    • Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
    Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2730) shows staining in U251 Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2730) at a dilution of 1:20 over night at 4C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
    Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2720) shows staining in MCF-7 Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2730) at a dilution of 1:20 over night at 4 C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
    Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2730) shows staining in Hela Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2720) at a dilution of 1:20 over night at 4 C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
    ICC/IF image of ab2730 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2730, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry - Anti-alpha Adaptin antibody [AP6] (ab2730)
    Flow Cytometry - Anti-alpha Adaptin antibody [AP6] (ab2730)

    Overlay histogram showing MCF7 cells stained with ab2730 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2730, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

プロトコール

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (21)

    ab2730 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2730 は 21 報の論文で使用されています。

    • Wagner W  et al. Myosin VI Drives Clathrin-Mediated AMPA Receptor Endocytosis to Facilitate Cerebellar Long-Term Depression. Cell Rep 28:11-20.e9 (2019). PubMed: 31269433
    • Charnley M  et al. A new role for Notch in the control of polarity and asymmetric cell division of developing T cells. J Cell Sci 133:N/A (2019). PubMed: 31578237
    • Evergren E  et al. Eps15R and clathrin regulate EphB2-mediated cell repulsion. Traffic 19:44-57 (2018). PubMed: 28972287
    • Hugo SE & Schlegel A A Genetic Model to Study Increased Hexosamine Biosynthetic Flux. Endocrinology 158:2420-2426 (2017). PubMed: 28582574
    • Humphries AC  et al. Cdc42 and the Rho GEF intersectin-1 collaborate with Nck to promote N-WASP-dependent actin polymerisation. J Cell Sci 127:673-85 (2014). PubMed: 24284073
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 14 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Adaptin antibody [AP6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (undifferentiated Human ES cells on MEFs)
    Permeabilization
    Yes - in blocking buffer
    Specification
    undifferentiated Human ES cells on MEFs
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 01 2016

    Western blot abreview for Anti-alpha Adaptin antibody [AP6]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Undifferentiated Human ES cells on MEFs)
    Gel Running Conditions
    Non-reduced Denaturing (12.5%)
    Loading amount
    20 µg
    Specification
    Undifferentiated Human ES cells on MEFs
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 25 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Adaptin antibody [AP6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEPG2)
    Specification
    HEPG2
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 18 2015

    Western blot abreview for Anti-alpha Adaptin antibody [AP6]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEPG2)
    Gel Running Conditions
    Reduced Denaturing (12.5)
    Loading amount
    20 µg
    Specification
    HEPG2
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 13 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Adaptin [AP6] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    0.1% BSA and 1% serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
    Sample
    Mouse Cell (mouse embryonic stem cell)
    Specification
    mouse embryonic stem cell
    Permeabilization
    Yes - 0.1% triton x
    Fixative
    methanol/acetone
    Read More

    Abcam user community

    Verified customer

    投稿 May 19 2014

    Western blot abreview for Anti-alpha Adaptin antibody [AP6]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    100000 cells
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cells)
    Specification
    mouse embryonic stem cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 09 2014

    Immunocytochemistry/ Immunofluorescence

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.2% Triton in PBS
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 05 2013

    Question

    Thank you for your reply. Since we got AP2 antibody from sigma and that antibody works, we did not really do trouble-shooting. About the refund, we always buy antibody from your company, so I am thinking that the active code that can be used at next purchase for the same price products may be good for us. Thank you.Best

    Read More

    Abcam community

    Verified customer

    Asked on Dec 21 2012

    Answer

    Thank you for contacting us.

    Your credit note ID is XXXXX.

    I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    The credit note ID is for your reference only and does not automatically guarantee the credit.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    Abcam Scientific Support

    Answered on Dec 21 2012

    Question

    ReviewID: 33690 AbID: 2730, Anti-alpha Adaptin antibody [AP6] Rating: Inconclusive Image: Anti-alpha Adaptin antibody [AP6] for Western blot (Mouse) Sample: Species: Mouse Type: Cell lysate - whole cell Loading amount: 90 µg Specification: mouse neuro2A Application: Application: Gel Running Conditions: Non-reduced Non-Denaturing (Native) (8% running gel) Blocking step: Milk as blocking agent for 1 hour at 25°C Blocking Concentration: 5% Other detail: Dilution: 1/300 Incubation time: 12 hours at 4°C Secondary Antibody: Name: Non-Abcam Antibody was used: anti-mouse IgG(whole molecule)-peroxidase, antibod Conjugation: HRP Dilution: 1/5000 Detection: Detection method: ECL Exposure: 30 minutes and 0 seconds Bands: Specific: no kDa Non-specific: too many kDa Positive Control: we use sigma A4325 monoclonal anti-a-adaptin (AP2)as a control,same sample solubilization, it is able to detecting AP2 pretty well. Additional Data:

    Read More

    Abcam community

    Verified customer

    Asked on Dec 21 2012

    Answer

    Thank you for submitting an Abreview of ab2730. As you may have noticed, your review has now been published on our website.

    Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

    Based on the information in your review, I feel that altering few step in your protocol may help improve your results. I wonder if you have tried troubleshooting the protocol. If yes, could you provide details.

    This antibody is covered by our Abpromise, so if the antibody was purchased within the past 6 months, I would be happy to discuss a refund or replacement with you to resolve this issue.

    I look forward to your reply.

    Read More

    Abcam Scientific Support

    Answered on Dec 21 2012

    Western blot abreview for Anti-alpha Adaptin antibody [AP6]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse neuro2A)
    Loading amount
    90 µg
    Specification
    mouse neuro2A
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (8% running gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 21 2012

    1-10 of 14 Abreviews or Q&A

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