製品の概要
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製品名Anti-alpha Adaptin antibody [AC1-M11]
alpha Adaptin 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [AC1-M11] to alpha Adaptin
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由来種Mouse
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アプリケーション適用あり: IP, WB, ICC/IF, ELISA, IHC-P, Flow Cytmore details
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種交差性交差種: Mouse, Rat, Chicken, Cow, Human, Pig, Non human primates, Amphibian
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免疫原
Full length native protein (purified) corresponding to Cow alpha Adaptin. Purified Bovine brain adaptor complexes
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ポジティブ･コントロール
- pig brain lysate and transfected COS cells.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度Protein A purified
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ポリ/モノモノクローナル
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クローン名AC1-M11
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アイソタイプIgG2a
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研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab2807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | 1/100. Detects a band of approximately 100 kDa. In pig brain extract detects a doublet of approximately 100 kDa representing the two isoforms of alpha-adaptin. | |
| ICC/IF | 1/100. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | 1/20. | |
| Flow Cyt | 1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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関連性Clathrin mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down regulated. During formation of clathrin coated membranes, clathrin co assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.
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細胞内局在Cytoplasmic
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参照データベース
- Entrez Gene: 515216 Cow
- Entrez Gene: 784636 Cow
- Entrez Gene: 160 Human
- Entrez Gene: 161 Human
- Entrez Gene: 11771 Mouse
- Entrez Gene: 11772 Mouse
- Entrez Gene: 308578 Rat
- Entrez Gene: 81637 Rat
see all -
別名
- 100 kDa coated vesicle protein A antibody
- 100 kDa coated vesicle protein C antibody
- Adapter related protein complex 2 subunit alpha 1 antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)](https://www.abcam.co.jp/ps/products/2/ab2807/Images/ab2807-5-ab2807IHC11.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)Immunohistochemistry was performed on normal biopsies of deparaffinized Human heart tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse monoclonal antibody recognizing alpha Adaptin (ab2807) or without primary antibody (negative control) overnight at 4�C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
![Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)](https://www.abcam.co.jp/ps/products/2/ab2807/Images/ab2807_1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)Immunolocalization of alpha-adaptin in NRK cells using ab2807. -
![Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)](https://www.abcam.co.jp/ps/products/2/ab2807/Images/ab2807_2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)Immunolocalization of alpha-adaptin in NRK cells using ab2807 (low power image of image 2).
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![Western blot - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)](https://www.abcam.co.jp/ps/products/2/ab2807/Images/alpha-Adaptin-Primary-antibodies-ab2807-2.jpg)
Western blot - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)This image is a courtesy of Anonymous AbreviewAnti-alpha Adaptin antibody [AC1-M11] (ab2807) at 1/500 dilution + Lysate was prepared from mouse neuroblastoma cells at 10 µg
Secondary
HRP-conjugated donkey monoclonal to mouse IgG at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 100 kDa (why is the actual band size different from the predicted?)
Exposure time: 10 seconds
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![Flow Cytometry - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)](https://www.abcam.co.jp/ps/products/2/ab2807/Images/alpha-Adaptin-Primary-antibodies-ab2807-3.jpg)
Flow Cytometry - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)Overlay histogram showing HepG2 cells stained with ab2807 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2807, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
参考文献
This product has been referenced in:
- Du M et al. A novel subcellular machine contributes to basal junction remodeling in the seminiferous epithelium. Biol Reprod 88:60 (2013). Read more (PubMed: 23303684) »
- Bogdanovic O et al. Numb/Numbl-Opo antagonism controls retinal epithelium morphogenesis by regulating integrin endocytosis. Dev Cell 23:782-95 (2012). Read more (PubMed: 23041384) »
レビューと Q&A
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