Anti-alpha Adaptin 抗体 [AC1-M11] (ab2807)
![Western blot - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)](https://www.abcam.co.jp/ps/products/2/ab2807/Images/ab2807-464472-Antialpha-Adaptin-antibody-AC1M11-wb.jpg "Western blot - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)")
Key features and details
- Mouse monoclonal [AC1-M11] to alpha Adaptin
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-alpha Adaptin antibody [AC1-M11]
alpha Adaptin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [AC1-M11] to alpha Adaptin -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, IHC-P, Flow Cytmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Full length native protein (purified) corresponding to Cow alpha Adaptin. Purified Bovine brain adaptor complexes
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ポジティブ･コントロール
- pig brain lysate and transfected COS cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
AC1-M11 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2807の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 108 kDa.
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| ICC/IF |
1/100.
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| IHC-P |
1/20.
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| Flow Cyt |
1/100.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| 特記事項 |
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WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 108 kDa. |
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ICC/IF
1/100. |
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IHC-P
1/20. |
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Flow Cyt
1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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関連性
Clathrin mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down regulated. During formation of clathrin coated membranes, clathrin co assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 160 Human
- Entrez Gene: 161 Human
- Entrez Gene: 11771 Mouse
- Entrez Gene: 11772 Mouse
- Entrez Gene: 308578 Rat
- Entrez Gene: 81637 Rat
- Omim: 601026 Human
- Omim: 607242 Human
see all -
別名
- 100 kDa coated vesicle protein A antibody
- 100 kDa coated vesicle protein C antibody
- Adapter related protein complex 2 subunit alpha 1 antibody
see all
画像
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Western blot - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)All lanes : Anti-alpha Adaptin antibody [AC1-M11] (ab2807) at 2 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : COLO 205 (Human colon adenocarcinoma cell line) whole cell lysate
Lane 5 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 7 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 8 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution
Predicted band size: 108 kDaKnown quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with Pierce™ Power Blotter System (22834). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)](https://www.abcam.co.jp/ps/products/2/ab2807/Images/ab2807-200556-anti-alpha-adaptin-antibody-ac1-m11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)Immunohistochemistry was performed on normal biopsies of deparaffinized Human heart tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse monoclonal antibody recognizing alpha Adaptin (ab2807) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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![Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)](https://www.abcam.co.jp/ps/products/2/ab2807/Images/ab2807_1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)Immunolocalization of alpha-adaptin in NRK cells using ab2807. -
![Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)](https://www.abcam.co.jp/ps/products/2/ab2807/Images/ab2807-6462-anti-alpha-adaptin-antibody-ac1-m11-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)Immunolocalization of alpha-adaptin in NRK cells using ab2807 (low power image of image 2).
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![Flow Cytometry - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)](https://www.abcam.co.jp/ps/products/2/ab2807/Images/ab2807-6460-anti-alpha-adaptin-antibody-ac1-m11-flow-cytometry.jpg)
Flow Cytometry - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)Overlay histogram showing HepG2 cells stained with ab2807 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2807, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab2807 は 10 報の論文で使用されています。
- Moulay G et al. Alternative splicing of clathrin heavy chain contributes to the switch from coated pits to plaques. J Cell Biol 219:N/A (2020). PubMed: 32642759
- Joseph JG et al. Complimentary action of structured and unstructured domains of epsin supports clathrin-mediated endocytosis at high tension. Commun Biol 3:743 (2020). PubMed: 33293652
- Baschieri F et al. Frustration of endocytosis potentiates compression-induced receptor signaling. J Cell Sci 133:N/A (2020). PubMed: 32788230
- Qureshi R et al. A Prokineticin-Driven Epigenetic Switch Regulates Human Epicardial Cell Stemness and Fate. Stem Cells 36:1589-1602 (2018). PubMed: 29873146
- Baschieri F et al. Frustrated endocytosis controls contractility-independent mechanotransduction at clathrin-coated structures. Nat Commun 9:3825 (2018). PubMed: 30237420
- Simsek D et al. The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Cell 169:1051-1065.e18 (2017). PubMed: 28575669
- Du M et al. A novel subcellular machine contributes to basal junction remodeling in the seminiferous epithelium. Biol Reprod 88:60 (2013). PubMed: 23303684
- Bogdanovic O et al. Numb/Numbl-Opo antagonism controls retinal epithelium morphogenesis by regulating integrin endocytosis. Dev Cell 23:782-95 (2012). PubMed: 23041384
- Gautier JJ et al. Clathrin is required for Scar/Wave-mediated lamellipodium formation. J Cell Sci 124:3414-27 (2011). ICC/IF ; Drosophila melanogaster . PubMed: 22010197
- Robinson MS 100-kD coated vesicle proteins: molecular heterogeneity and intracellular distribution studied with monoclonal antibodies. J Cell Biol 104:887-95 (1987). PubMed: 2881934