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    alpha-adaptin-antibody-ac1-m11-ab2807.pdf

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Signal Transduction Adapters Transmembrane
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Anti-alpha Adaptin 抗体 [AC1-M11] (ab2807)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (10)

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Western blot - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
  • Flow Cytometry - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)

Key features and details

  • Mouse monoclonal [AC1-M11] to alpha Adaptin
  • Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

こちらの製品もご検討ください

Biochemical
Product image
Latrunculin A (LAT-A), Actin polymerization inhibitor (ab144290)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
一次抗体
Product image
Anti-Clathrin heavy chain antibody (ab21679)

関連製品

製品の概要

  • 製品名

    Anti-alpha Adaptin antibody [AC1-M11]
    alpha Adaptin 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [AC1-M11] to alpha Adaptin
  • 由来種

    Mouse
  • アプリケーション

    適用あり: WB, ICC/IF, IHC-P, Flow Cytmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Full length native protein (purified) corresponding to Cow alpha Adaptin. Purified Bovine brain adaptor complexes

  • ポジティブ・コントロール

    • pig brain lysate and transfected COS cells.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    AC1-M11
  • アイソタイプ

    IgG2a
  • 研究分野

    • Signal Transduction
    • Adapters
    • Transmembrane

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2807の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
Use a concentration of 2 µg/ml. Predicted molecular weight: 108 kDa.
ICC/IF
1/100.
IHC-P
1/20.
Flow Cyt
1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

特記事項
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 108 kDa.
ICC/IF
1/100.
IHC-P
1/20.
Flow Cyt
1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 関連性

    Clathrin mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down regulated. During formation of clathrin coated membranes, clathrin co assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.
  • 細胞内局在

    Cytoplasmic
  • 参照データベース

    • Entrez Gene: 160 Human
    • Entrez Gene: 161 Human
    • Entrez Gene: 11771 Mouse
    • Entrez Gene: 11772 Mouse
    • Entrez Gene: 308578 Rat
    • Entrez Gene: 81637 Rat
    • Omim: 601026 Human
    • Omim: 607242 Human
    • SwissProt: O94973 Human
    • SwissProt: O95782 Human
    • SwissProt: P17426 Mouse
    • SwissProt: P17427 Mouse
    • SwissProt: P18484 Rat
    see all
  • 別名

    • 100 kDa coated vesicle protein A antibody
    • 100 kDa coated vesicle protein C antibody
    • Adapter related protein complex 2 subunit alpha 1 antibody
    • Adapter related protein complex 2 subunit alpha 2 antibody
    • Adaptin alpha A antibody
    • Adaptin alpha B antibody
    • Adaptin, alpha B antibody
    • Adaptor protein complex AP 2 alpha 1 subunit antibody
    • Adaptor protein complex AP 2 alpha 2 subunit antibody
    • Adaptor protein complex AP 2 subunit alpha 1 antibody
    • Adaptor related protein complex 2 alpha 1 subunit antibody
    • Adaptor related protein complex 2 alpha 2 subunit antibody
    • ADTAA antibody
    • ADTAB antibody
    • Alpha adaptin A antibody
    • Alpha adaptin C antibody
    • Alpha adaptin C; Huntingtin interacting protein J antibody
    • Alpha1 adaptin antibody
    • Alpha2 adaptin antibody
    • AP 2 complex subunit alpha 1 antibody
    • AP 2 complex subunit alpha 2 antibody
    • AP2 alpha antibody
    • AP2A1 antibody
    • AP2A2 antibody
    • CLAPA1 antibody
    • CLAPA2 antibody
    • Clathrin assembly protein complex 2 alpha A large chain antibody
    • Clathrin assembly protein complex 2 alpha C large chain antibody
    • Clathrin associated/assembly/adaptor protein large alpha 1 antibody
    • Clathrin associated/assembly/adaptor protein large alpha 2 antibody
    • HIP 9 antibody
    • HIP9 antibody
    • Huntingtin interacting protein antibody
    • Huntingtin interacting protein HYPJ antibody
    • Huntingtin interacting protein J antibody
    • Huntingtin yeast partner J antibody
    • HYPJ antibody
    • Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody
    • Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit antibody
    see all

画像

  • Western blot - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
    Western blot - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
    All lanes : Anti-alpha Adaptin antibody [AC1-M11] (ab2807) at 2 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 4 : COLO 205 (Human colon adenocarcinoma cell line) whole cell lysate
    Lane 5 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 7 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 8 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution

    Predicted band size: 108 kDa



    Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with Pierce™ Power Blotter System (22834). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human heart tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse monoclonal antibody recognizing alpha Adaptin (ab2807) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
    Immunolocalization of alpha-adaptin in NRK cells using ab2807.
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)

    Immunolocalization of alpha-adaptin in NRK cells using ab2807 (low power image of image 2).

  • Flow Cytometry - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
    Flow Cytometry - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
    Overlay histogram showing HepG2 cells stained with ab2807 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2807, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

プロトコール

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (10)

ab2807 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2807 は 10 報の論文で使用されています。

  • Moulay G  et al. Alternative splicing of clathrin heavy chain contributes to the switch from coated pits to plaques. J Cell Biol 219:N/A (2020). PubMed: 32642759
  • Joseph JG  et al. Complimentary action of structured and unstructured domains of epsin supports clathrin-mediated endocytosis at high tension. Commun Biol 3:743 (2020). PubMed: 33293652
  • Baschieri F  et al. Frustration of endocytosis potentiates compression-induced receptor signaling. J Cell Sci 133:N/A (2020). PubMed: 32788230
  • Qureshi R  et al. A Prokineticin-Driven Epigenetic Switch Regulates Human Epicardial Cell Stemness and Fate. Stem Cells 36:1589-1602 (2018). PubMed: 29873146
  • Baschieri F  et al. Frustrated endocytosis controls contractility-independent mechanotransduction at clathrin-coated structures. Nat Commun 9:3825 (2018). PubMed: 30237420
  • Simsek D  et al. The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Cell 169:1051-1065.e18 (2017). PubMed: 28575669
  • Du M  et al. A novel subcellular machine contributes to basal junction remodeling in the seminiferous epithelium. Biol Reprod 88:60 (2013). PubMed: 23303684
  • Bogdanovic O  et al. Numb/Numbl-Opo antagonism controls retinal epithelium morphogenesis by regulating integrin endocytosis. Dev Cell 23:782-95 (2012). PubMed: 23041384
  • Gautier JJ  et al. Clathrin is required for Scar/Wave-mediated lamellipodium formation. J Cell Sci 124:3414-27 (2011). ICC/IF ; Drosophila melanogaster . PubMed: 22010197
  • Robinson MS 100-kD coated vesicle proteins: molecular heterogeneity and intracellular distribution studied with monoclonal antibodies. J Cell Biol 104:887-95 (1987). PubMed: 2881934

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Western blot abreview for Anti-alpha Adaptin antibody [AC1-M11]

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Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Dog Cell lysate - other (MDCK cells)
Gel Running Conditions
Reduced Denaturing (10 % SDS gel)
Loading amount
10 µg
Specification
MDCK cells
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 21°C
Read More

Abcam user community

Verified customer

投稿 Jan 03 2019

Western blot abreview for Anti-alpha Adaptin antibody [AC1-M11]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Neuroblastoma)
Loading amount
10 µg
Specification
Neuroblastoma
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

投稿 Nov 10 2009

Question

Hello,

 

I would like to inquire about the concentration of an antibody solution that we had purchased. Could you please let me know the concentration of Anti-alpha Adaptin antibody [AC1-M11], catalogue number ab2807, Lot GR21203-4?

 

Thank you very much,

 

Min Du

Vogl Lab

University of British Columbia

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Abcam community

Verified customer

Asked on Aug 01 2012

Answer

Thank you for contacting us.

The concentration of this lot is 2mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Abcam Scientific Support

Answered on Aug 01 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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