Anti-alpha Adaptin 抗体 [AC1-M11] (ab2807)

Reviews (2)Q&A (1)References (6)

Key features and details

  • Mouse monoclonal [AC1-M11] to alpha Adaptin
  • Suitable for: ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Chicken, Cow, Human, Pig, Non human primates, Amphibian
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-alpha Adaptin antibody [AC1-M11]
    alpha Adaptin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [AC1-M11] to alpha Adaptin

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ICC/IF, IHC-P, Flow Cytmore details

  • 種交差性

    交差種: Mouse, Rat, Chicken, Cow, Human, Pig, Non human primates, Amphibian

  • 免疫原

    Full length native protein (purified) corresponding to Cow alpha Adaptin. Purified Bovine brain adaptor complexes

  • ポジティブ･コントロール

    • pig brain lysate and transfected COS cells.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    AC1-M11

  • アイソタイプ

    IgG2a

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100.
IHC-P 1/20.
Flow Cyt 1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 関連性

    Clathrin mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down regulated. During formation of clathrin coated membranes, clathrin co assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.

  • 細胞内局在

    Cytoplasmic

  • 参照データベース

    see all

  • 別名

    • 100 kDa coated vesicle protein A antibody
    • 100 kDa coated vesicle protein C antibody
    • Adapter related protein complex 2 subunit alpha 1 antibody
    • Adapter related protein complex 2 subunit alpha 2 antibody
    • Adaptin alpha A antibody
    • Adaptin alpha B antibody
    • Adaptin, alpha B antibody
    • Adaptor protein complex AP 2 alpha 1 subunit antibody
    • Adaptor protein complex AP 2 alpha 2 subunit antibody
    • Adaptor protein complex AP 2 subunit alpha 1 antibody
    • Adaptor related protein complex 2 alpha 1 subunit antibody
    • Adaptor related protein complex 2 alpha 2 subunit antibody
    • ADTAA antibody
    • ADTAB antibody
    • Alpha adaptin A antibody
    • Alpha adaptin C antibody
    • Alpha adaptin C; Huntingtin interacting protein J antibody
    • Alpha1 adaptin antibody
    • Alpha2 adaptin antibody
    • AP 2 complex subunit alpha 1 antibody
    • AP 2 complex subunit alpha 2 antibody
    • AP2 alpha antibody
    • AP2A1 antibody
    • AP2A2 antibody
    • CLAPA1 antibody
    • CLAPA2 antibody
    • Clathrin assembly protein complex 2 alpha A large chain antibody
    • Clathrin assembly protein complex 2 alpha C large chain antibody
    • Clathrin associated/assembly/adaptor protein large alpha 1 antibody
    • Clathrin associated/assembly/adaptor protein large alpha 2 antibody
    • HIP 9 antibody
    • HIP9 antibody
    • Huntingtin interacting protein antibody
    • Huntingtin interacting protein HYPJ antibody
    • Huntingtin interacting protein J antibody
    • Huntingtin yeast partner J antibody
    • HYPJ antibody
    • Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody
    • Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human heart tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse monoclonal antibody recognizing alpha Adaptin (ab2807) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
    Immunolocalization of alpha-adaptin in NRK cells using ab2807.
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)

    Immunolocalization of alpha-adaptin in NRK cells using ab2807 (low power image of image 2).

  • Flow Cytometry - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
    Flow Cytometry - Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
    Overlay histogram showing HepG2 cells stained with ab2807 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2807, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

参考文献 (6)

ab2807 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2807 は 6 報の論文で使用されています。

  • Baschieri F  et al. Frustrated endocytosis controls contractility-independent mechanotransduction at clathrin-coated structures. Nat Commun 9:3825 (2018). PubMed: 30237420
  • Simsek D  et al. The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Cell 169:1051-1065.e18 (2017). PubMed: 28575669
  • Du M  et al. A novel subcellular machine contributes to basal junction remodeling in the seminiferous epithelium. Biol Reprod 88:60 (2013). PubMed: 23303684
  • Bogdanovic O  et al. Numb/Numbl-Opo antagonism controls retinal epithelium morphogenesis by regulating integrin endocytosis. Dev Cell 23:782-95 (2012). PubMed: 23041384
  • Gautier JJ  et al. Clathrin is required for Scar/Wave-mediated lamellipodium formation. J Cell Sci 124:3414-27 (2011). ICC/IF ; Drosophila melanogaster . PubMed: 22010197
  • Robinson MS 100-kD coated vesicle proteins: molecular heterogeneity and intracellular distribution studied with monoclonal antibodies. J Cell Biol 104:887-95 (1987). PubMed: 2881934

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Western blot abreview for Anti-alpha Adaptin antibody [AC1-M11]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Dog Cell lysate - other (MDCK cells)
Gel Running Conditions
Reduced Denaturing (10 % SDS gel)
Loading amount
10 µg
Specification
MDCK cells
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 21°C
Read More

Abcam user community

Verified customer

投稿 Jan 03 2019

Question

Hello,

 

I would like to inquire about the concentration of an antibody solution that we had purchased. Could you please let me know the concentration of Anti-alpha Adaptin antibody [AC1-M11], catalogue number ab2807, Lot GR21203-4?

 

Thank you very much,

 

Min Du

Vogl Lab

University of British Columbia

Read More
Answer

Thank you for contacting us.

The concentration of this lot is 2mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Western blot abreview for Anti-alpha Adaptin antibody [AC1-M11]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Neuroblastoma)
Loading amount
10 µg
Specification
Neuroblastoma
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

投稿 Nov 10 2009

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