Application
Western blot
Sample
Rat Cell lysate - other (Primary lung epithelia cells)
Gel Running Conditions
Reduced Denaturing (4-12% Nupage)
Specification
Primary lung epithelia cells
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 22°C
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Western Breeze Life Technologies
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Detection
Detection method
Chromogenic
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 104 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Nov 02 2015