Anti-alpha Actinin 4 抗体 (ab96686)
Key features and details
- Rabbit polyclonal to alpha Actinin 4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-alpha Actinin 4 antibody
alpha Actinin 4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to alpha Actinin 4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Zebrafish
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免疫原
Recombinant fragment containing a sequence corresponding to a region within amino acids 206-512 of Human alpha Actinin 4 (AAH05033).
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ポジティブ･コントロール
- 293T, A431, H1299, HepG2 whole cell lysates HeLa cells; A549 xenograft (IHC)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96686の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 105 kDa.
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| IHC-P |
1/100 - 1/500.
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| ICC/IF |
1/100 - 1/200.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 105 kDa. |
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IHC-P
1/100 - 1/500. |
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ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. -
組織特異性
Widely expressed. -
関連疾患
Defects in ACTN4 are the cause of focal segmental glomerulosclerosis type 1 (FSGS1) [MIM:603278]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. -
配列類似性
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
細胞内局在
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines. - Information by UniProt
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参照データベース
- Entrez Gene: 522269 Cow
- Entrez Gene: 81 Human
- Entrez Gene: 60595 Mouse
- Entrez Gene: 63836 Rat
- Entrez Gene: 322221 Zebrafish
- Omim: 604638 Human
- SwissProt: A5D7D1 Cow
- SwissProt: O43707 Human
see all -
別名
- actinin 4 antibody
- Actinin alpha 4 antibody
- actinin4 antibody
see all
画像
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Western blot - Anti-alpha Actinin 4 antibody (ab96686)All lanes : Anti-alpha Actinin 4 antibody (ab96686) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 105 kDa
7.5% SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin 4 antibody (ab96686)ab96686, at 1/200 dilution, staining names alpha Actinin 4 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody (ab96686)ab96686, at 1/500 dilution, staining alpha Actinin 4 in paraffin-embedded A549 xenograft by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab96686 は 1 報の論文で使用されています。
- Kalendová A et al. Nuclear actin filaments recruit cofilin and actin-related protein 3, and their formation is connected with a mitotic block. Histochem Cell Biol 142:139-52 (2014). PubMed: 25002125