Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4432] to alpha 2 Macroglobulin - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
製品名Anti-alpha 2 Macroglobulin antibody [EPR4432] - BSA and Azide free
alpha 2 Macroglobulin 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR4432] to alpha 2 Macroglobulin - BSA and Azide free
アプリケーション適用あり: IHC-P, WBmore details
適用なし: Flow Cyt,ICC or IP
Synthetic peptide within Human alpha 2 Macroglobulin aa 1300-1400. The exact sequence is proprietary.
ab247855 is the carrier-free version of ab109422 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247855 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab247855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 163 kDa.|
機能Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
組織特異性Secreted in plasma.
配列類似性Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
発生段階Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
- Information by UniProt
- A2m antibody
- A2MG_HUMAN antibody
- Alpha 2 M antibody
This data was developed using ab109422, the same antibody clone in a different buffer formulation.
ab109422, at 1/500 dilution, staining alpha 2 Macroglobulin in paraffin-embedded Human liver by Immunohistochemistry.Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.