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Anti-alpha 1 Spectrin 抗体 [17C7] (ab11751)

Reviews (3)Q&A (1)References (12)
Western blot - Anti-alpha 1 Spectrin antibody [17C7] (ab11751)
  • Western blot - Anti-alpha 1 Spectrin antibody [17C7] (ab11751)

Key features and details

  • Mouse monoclonal [17C7] to alpha 1 Spectrin
  • Suitable for: ELISA, IHC-Fr, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-alpha 1 Spectrin antibody [17C7]
    alpha 1 Spectrin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [17C7] to alpha 1 Spectrin

  • 由来種

    Mouse

  • 特異性

    ab11751 is developed against the alpha I spectrin SH3 domain. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In NIH 3T3 cells, ab11751 reacts with a 200 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes.

  • アプリケーション

    適用あり: ELISA, IHC-Fr, ICC/IF, WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant alpha I spectrin (erythroid spectrin) SH3 domain.

  • ポジティブ･コントロール

    • Erythroid spectrin in red blood cells and cerebellum.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab11751の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF (2)
Use at an assay dependent concentration.

See Abreview.
WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 280 kDa.
特記事項
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

See Abreview.
WB
Use at an assay dependent concentration. Predicted molecular weight: 280 kDa.

ターゲット情報

  • 機能

    Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.

  • 関連疾患

    Defects in SPTA1 are the cause of elliptocytosis type 2 (EL2) [MIM:130600]. EL2 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
    Defects in SPTA1 are a cause of hereditary pyropoikilocytosis (HPP) [MIM:266140]. HPP is an autosomal recessive disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells.
    Defects in SPTA1 are the cause of spherocytosis type 3 (SPH3) [MIM:270970]; also known as hereditary spherocytosis type 3 (HS3). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH3 is characterized by severe hemolytic anemia. Inheritance is autosomal recessive.

  • 配列類似性

    Belongs to the spectrin family.
    Contains 3 EF-hand domains.
    Contains 1 SH3 domain.
    Contains 21 spectrin repeats.

  • 細胞内局在

    Cytoplasm > cytoskeleton. Cytoplasm > cell cortex.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Alpha I spectrin antibody
      • EL 2 antibody
      • EL2 antibody
      • Elliptocytosis 2 antibody
      • Elliptocytosis2 antibody
      • Erythrocyte alpha spectrin antibody
      • erythrocyte antibody
      • Erythroid alpha spectrin antibody
      • Erythroid alpha-spectrin antibody
      • Erythroid spectrin alpha antibody
      • HPP antibody
      • HS3 antibody
      • Spectrin alpha chain antibody
      • Spectrin alpha chain erythrocyte antibody
      • Spectrin alpha erythrocytic 1 antibody
      • SPH3 antibody
      • SPTA 1 antibody
      • SPTA antibody
      • SPTA1 antibody
      • SPTA1_HUMAN antibody
      see all

    画像

    • Western blot - Anti-alpha 1 Spectrin antibody [17C7] (ab11751)
      Western blot - Anti-alpha 1 Spectrin antibody [17C7] (ab11751)
      All lanes : Anti-alpha 1 Spectrin antibody [17C7] (ab11751) at 1 µg/ml

      Lane 1 : MW marker
      Lane 2 : Human Lung lysate
      Lane 3 : Mouse Lung lysate
      Lane 4 : Rat Brain lysate
      Lane 5 : Human Kidney lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-mouse IgG

      Predicted band size: 280 kDa

    • Western blot - Anti-alpha 1 Spectrin antibody [17C7] (ab11751)
      Western blot - Anti-alpha 1 Spectrin antibody [17C7] (ab11751)This image is courtesy of an Abreview submitted by Dr. Mahesh Shivananjappa
      Anti-alpha 1 Spectrin antibody [17C7] (ab11751) at 1/1000 dilution + Human Platelet whole cell lysate at 25 µg

      Secondary
      HRP-conjugated Goat Anti-mouse IgG at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 280 kDa
      Additional bands at: 200 kDa (possible cleavage fragment)


      Exposure time: 1 hour

      See Abreview

    参考文献 (12)

    ab11751 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab11751 は 12 報の論文で使用されています。

    • Dekel E  et al. 20S proteasomes secreted by the malaria parasite promote its growth. Nat Commun 12:1172 (2021). PubMed: 33608523
    • Smith AS  et al. MYH9-related disease mutations cause abnormal red blood cell morphology through increased myosin-actin binding at the membrane. Am J Hematol 94:667-677 (2019). PubMed: 30916803
    • Wang Y  et al. Critical roles of aII spectrin in brain development and epileptic encephalopathy. J Clin Invest 128:760-773 (2018). PubMed: 29337302
    • Huang HM  et al. Ankyrin-1 Gene Exhibits Allelic Heterogeneity in Conferring Protection Against Malaria. G3 (Bethesda) 7:3133-3144 (2017). WB ; Mouse . PubMed: 28751503
    • Olumuyiwa-Akeredolu OO  et al. Characterizing pathology in erythrocytes using morphological and biophysical membrane properties: Relation to impaired hemorheology and cardiovascular function in rheumatoid arthritis. Biochim Biophys Acta Biomembr 1859:2381-2391 (2017). PubMed: 28919343
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha 1 Spectrin antibody [17C7]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (rat artery smooth muscle cells)
    Specification
    rat artery smooth muscle cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton x100
    Read More

    Dr. Rosalind Silverman-Gavrila

    Verified customer

    投稿 Jan 20 2012

    Western blot abreview for Anti-alpha 1 Spectrin antibody [17C7]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Platelet Lysate (precleared))
    Loading amount
    25 µg
    Specification
    Platelet Lysate (precleared)
    Gel Running Conditions
    Reduced Denaturing (4-15)
    Blocking step
    Thermo Starting Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
    Read More

    Dr. Mahesh Shivananjappa

    Verified customer

    投稿 Jan 08 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha 1 Spectrin antibody [17C7]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (PMN)
    Specification
    PMN
    Fixative
    Paraformaldehyde
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Read More

    Dr. Mahesh Shivananjappa

    Verified customer

    投稿 Dec 22 2009

    Question

    I have a question about this antibody "Anti-alpha 1 Spectrin antibody [17C7] (ab11751)": does the antiboby work on the paraffin sections?

    Best Regards,

    Read More
    Answer

    Thank you for your enquiry.

    I am sorry to confirm that as far as we are aware, ab11751 has never been tested in immunohisochemistry on paraffin embedded sections (IHC-P). All tested and guaranteed applications and speciesare specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

    If you would like to test the antibody in this untested application, please contact me agian prior to the purchase by replying to this message as you may be eligible for our testing discount program.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

    Read More

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