Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2375(2)] to alpha 1 Sodium Potassium ATPase (phospho Y10) - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
製品名Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] - BSA and Azide free
alpha 1 Sodium Potassium ATPase 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR2375(2)] to alpha 1 Sodium Potassium ATPase (phospho Y10) - BSA and Azide free
This antibody only detects alpha 1 Sodium Potassium ATPase phosphorylated at Tyrosine 10.
アプリケーション適用あり: WBmore details
適用なし: Flow Cyt,ICC or IHC-P
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human alpha 1 Sodium Potassium ATPase (phospho Y10). The exact sequence is proprietary.
Ab247956 is the carrier-free version of ab124677. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247956 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
Concentration information loading...
Our Abpromise guarantee covers the use of ab247956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 113 kDa).|
機能This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
配列類似性Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
翻訳後修飾Phosphorylation on Tyr-10 modulates pumping activity.
細胞内局在Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- A1A1 antibody
- AT1A1 antibody
- AT1A1_HUMAN antibody
All lanes : Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] (ab124677) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate, treated with Pervanadate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 113 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
This data was developed using ab124677, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.