Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5880] to Alpha 1 microglobulin - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
製品名Anti-Alpha 1 microglobulin antibody [EPR5880] - BSA and Azide free
Alpha 1 microglobulin 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5880] to Alpha 1 microglobulin - BSA and Azide free
アプリケーション適用あり: IHC-P, WBmore details
Synthetic peptide within Human Alpha 1 microglobulin aa 50-150. The exact sequence is proprietary.
ab248270 is the carrier-free version of ab129059 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248270 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 1.17 x 10 -10 M Learn more about KD
Concentration information loading...
精製度Protein A purified
Our Abpromise guarantee covers the use of ab248270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 31 kDa (predicted molecular weight: 39 kDa).|
機能Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
Trypstatin is a trypsin inhibitor.
組織特異性Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
配列類似性In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
Contains 2 BPTI/Kunitz inhibitor domains.
翻訳後修飾The precursor is proteolytically processed into separately functioning proteins.
3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
Addition of glycosaminoglycan chondroitin sulfate, allows cross-linking between the different components.
- Information by UniProt
- A1M antibody
- Alpha 1 microglobulin/bikunin precursor antibody
- Alpha 1 microglycoprotein antibody
This data was developed using ab129059, the same antibody clone in a different buffer formulation.
ab129059 at 1/50 dilution staining Bikunin + Trypstatin in paraffin-embedded Human liver tissue by Immunohistochemistry.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.