Anti-alpha 1 Fetoprotein 抗体 [RM1011] (ab284388)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [RM1011] to alpha 1 Fetoprotein
- Suitable for: IHC-P, Indirect ELISA, IP, ICC, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-alpha 1 Fetoprotein antibody [RM1011]
alpha 1 Fetoprotein 一次抗体 製品一覧 -
製品の詳細
Rabbit recombinant multiclonal [RM1011] to alpha 1 Fetoprotein -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, Indirect ELISA, IP, ICC, Flow Cyt, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers. -
ポジティブ・コントロール
- WB: HepG2, Huh7, Hepa1-6, Hepa1-6 treated with 300ng/ml BFA for 24h, and Mouse placenta, Rat placenta lysates. IHC-P: Human hepatic carcinoma. ICC: HepG2 cells, & Hepa1-6 cells treated with Brefeldin A (300 ng/ml) for 24 h. Flow Cyt: HepG2, & Hepa1-6 cells treated with 300ng/ml BFA for 24h. IP: HepG2 cells.
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特記事項
IHC suitable for Human only.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
Recombinant Multiclonal -
クローン名
RM1011 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab284388の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC suitable for Human only. |
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Indirect ELISA |
Use at an assay dependent concentration.
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IP |
1/30.
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ICC |
1/50.
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Flow Cyt |
1/500.
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WB |
1/1000. Predicted molecular weight: 68 kDa.
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特記事項 |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC suitable for Human only. |
Indirect ELISA
Use at an assay dependent concentration. |
IP
1/30. |
ICC
1/50. |
Flow Cyt
1/500. |
WB
1/1000. Predicted molecular weight: 68 kDa. |
ターゲット情報
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機能
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
組織特異性
Plasma. Synthesized by the fetal liver and yolk sac. -
配列類似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
発生段階
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
翻訳後修飾
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 174 Human
- Entrez Gene: 11576 Mouse
- Entrez Gene: 24177 Rat
- Omim: 104150 Human
- SwissProt: P02771 Human
- SwissProt: P02772 Mouse
- SwissProt: P02773 Rat
- Unigene: 518808 Human
see all -
別名
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
see all
画像
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This data was developed using ab284388, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling alpha 1 Fetoprotein with ab284388 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2µg/mL) dilution (Green). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5µg/mL dilution (Red). The Nuclear counterstain was DAPI (Blue).
Confocal image showing cytoplasmic staining in HepG2 cells is observed.
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/mL) dilution.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Hepa1-6 cells labelling alpha 1 Fetoprotein with ab284388 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2µg/mL dilution (Green). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5µg/mL) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Confocal image showing increased cytoplasmic staining in Hepa1-6 cells treated with Brefeldin A (300 ng/ml) for 24 h is observed.
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2µg/mL) dilution.
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Immunohistochemical analysis of paraffin-embedded Human hepatic carcinoma tissue labelling alpha 1 Fetoprotein with ab284388 at 1/2000 (0.231 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human hepatic carcinoma. The section was incubated with ab284388 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Negative control: No staining on human liver.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling alpha 1 Fetoprotein with ab284388 at 1/2000 (0.231 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab284388 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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All lanes : Anti-alpha 1 Fetoprotein antibody [RM1011] (ab284388) at 1/1000 dilution
Lane 1 : HepG2 (Human hepatocellar carcinoma epithelial cell) whole cell lysate
Lane 2 : Huh7 (Human hepatocellar carcinoma epithelial cell) whole cell lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysate
Lane 5 : Hepa1-6 treated with 300ng/ml BFA for 24h whole cell lysate
Lane 6 : Mouse placenta lysate
Lane 7 : Rat placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: MDA-MB-231.
Exposure time: Lane 4-5: 26 seconds ; Others: 5 seconds
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling alpha 1 Fetoprotein with ab284388 at 1/500 dilution Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized Hepa1-6 (Mouse hepatoma epithelial cell) treated with 300ng/ml BFA for 24h (Right) / Untreated control (Left) cells labelling alpha 1 Fetoprotein with ab284388 at 1/500 dilution. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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alpha 1 Fetoprotein was immunoprecipitated from 0.35 mg HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10ug with ab284388 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab284388 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10ug
Lane 2: abab284388 IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab284388 in HepG2 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds
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ELISA using ab284388 at varying antibody concentrations and antigen concentration at 1000 ng/ml. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab284388 は 1 報の論文で使用されています。
- Xue X et al. YAP ISGylation increases its stability and promotes its positive regulation on PPP by stimulating 6PGL transcription. Cell Death Discov 8:59 (2022). PubMed: 35149670