Application
Western blot
Sample
Human Cell lysate - whole cell (hES-derived hepatocytes)
Loading amount
10 µg
Specification
hES-derived hepatocytes
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse (Dako)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 62-65 kDa
Positive control
Lanes 5 and 6 contain hepatoblast lysates, lanes 7 and 8 contain hepatocyte lysates.
Negative control
Lanes 1 and 2 contain human embryonic stem (ES) cell lysates, lanes 3 and 4 contain definitive endoderm lysates.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 06 2013