Anti-alpha 1 Fetoprotein 抗体 [AFP-01] (ab3980)

Reviews (3)Q&A (5)References (30)
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)

    Key features and details

    • Mouse monoclonal [AFP-01] to alpha 1 Fetoprotein
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-alpha 1 Fetoprotein antibody [AFP-01]
      alpha 1 Fetoprotein 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [AFP-01] to alpha 1 Fetoprotein

    • 由来種

      Mouse

    • 特異性

      The antibody reacts with human alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.

    • アプリケーション

      適用あり: ICC/IFmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Full length native protein (purified) corresponding to Human alpha 1 Fetoprotein.

    • ポジティブ･コントロール

      • Hep G2 human hepatocellular carcinoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3980の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ICC/IF (2)
    Use a concentration of 5 µg/ml.
    特記事項
    ICC/IF
    Use a concentration of 5 µg/ml.

    ターゲット情報

    • 機能

      Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

    • 組織特異性

      Plasma. Synthesized by the fetal liver and yolk sac.

    • 配列類似性

      Belongs to the ALB/AFP/VDB family.
      Contains 3 albumin domains.

    • 発生段階

      Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.

    • 翻訳後修飾

      Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
      Sulfated.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Afp antibody
        • AFPD antibody
        • Alpha fetoglobulin antibody
        • Alpha fetoprotein antibody
        • Alpha fetoprotein precursor antibody
        • Alpha-1-fetoprotein antibody
        • Alpha-fetoglobulin antibody
        • Alpha-fetoprotein antibody
        • alpha-fetoprotein, Hereditary persistence of, included antibody
        • FETA antibody
        • FETA_HUMAN antibody
        • Hereditary persistence of alpha fetoprotein antibody
        • HPAFP antibody
        see all

      画像

      • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)
        Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)

        ICC/IF image of ab3980 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3980, 5µg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      参考文献 (30)

      ab3980 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab3980 は 30 報の論文で使用されています。

      • Lenz D  et al. Generation of an iPSC line from a patient with infantile liver failure syndrome 2 due to mutations in NBAS: DHMCi004-A. Stem Cell Res 35:101398 (2019). PubMed: 30772683
      • Wei J  et al. Establishment and characterization of the GC-030-35 cell line derived from gastric hepatoid adenocarcinoma. Cancer Manag Res 11:1275-1287 (2019). PubMed: 30799954
      • Jung-Klawitter S  et al. Generation of 2 iPSC clones from a patient with DNAJC12 deficiency: DHMCi003-A and DHMCi003-B. Stem Cell Res 36:101402 (2019). PubMed: 30901742
      • Lenz D  et al. Generation of an induced pluripotent stem cell (iPSC) line, DHMCi005-A, from a patient with CALFAN syndrome due to mutations in SCYL1. Stem Cell Res 37:101428 (2019). PubMed: 30959346
      • Tongkobpetch S  et al. Generation of two human iPSC lines (MDCUi001-A and MDCUi001-B) from dermal fibroblasts of a Thai patient with X-linked osteogenesis imperfecta using integration-free Sendai virus. Stem Cell Res 39:101493 (2019). PubMed: 31326747
      View all Publications for this product

      レビューと Q&A

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      1-8 of 8 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha 1 Fetoprotein antibody [AFP-01]

       Inconclusive
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (mES derived embryoid bodies)
      Permeabilization
      Yes - 0.05% triton
      Specification
      mES derived embryoid bodies
      Blocking step
      Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 20% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Orla Fannon

      Verified customer

      投稿 Sep 12 2017

      Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha 1 Fetoprotein antibody [AFP-01]

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HEK293)
      Permeabilization
      No
      Specification
      HEK293
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Acetone
      Read More

      Abcam user community

      Verified customer

      投稿 Aug 31 2015

      Western blot abreview for Anti-alpha 1 Fetoprotein antibody [AFP-01]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (hES-derived hepatocytes)
      Loading amount
      10 µg
      Specification
      hES-derived hepatocytes
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      投稿 Feb 06 2013

      Question

      I need a pair of anti alpha fetoprotein antibodies for ELISA. But I do not know which ones could work together. Can you give me some suggestion?

      Read More
      Answer

      Having reviewed our catalog of anti-alpha 1 Fetoprotein antibodies suitable for use in ELISA, I have found the following 2 pairs which have been verified by us. AB8276 pairs with ab37207. More information about these items may be found at: Ab8276: https://www.abcam.com/alpha-1-Fetoprotein-Receptor-antibody-5E1-ab8276.html Ab37207: https://www.abcam.com/alpha-1-Fetoprotein-Receptor-antibody-2B8-ab37207.html Another pair which have been verified to work with each other in ELISA are ab3980 and ab3969. More information about these products may be found at: Ab3980: https://www.abcam.com/alpha-1-Fetoprotein-antibody-AFP-01-ab3980.html Ab3969: https://www.abcam.com/alpha-1-Fetoprotein-antibody-AFP-11-ab3969.html

      Read More

      Question

      We have been using your goat anti-human AFP antibody and I noticed on your website that it is no longer sold in the US. Do you offer a substitute or have a recommendation on what to use instead of ab8202-1?

      Read More
      Answer

      I am sorry for the inconvenience caused by this import restriction. While ab3980 is our only Anti-alpha 1 Fetoprotein raised in goat, we do offer a number of other Anti-alpha 1 Fetoprotein. We would recommend use of ab3980, Mouse monoclonal [AFP-01] to alpha 1 Fetoprotein as an alternative to the restricted antibody. Ab3980 has been tested in many of the same applications and has the same immunogen. This product also has been referenced in four publications.

      Read More

      Question

      I am guessing I received a bad batch. In any case, we would like to use a different antibody to avoid further problems. I see that the Anti-alpha 1 Fetoprotein antibody [AFP-11] (ab3969) has been tested exactly in the application we need to use it. Could you send us this antibody? when could we expect to get it?

      Kind regards

      Read More
      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1098413.

      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      PS: The product will be dispatched tomorrow for Friday delivery.

      Read More

      Question

      yIn a rush and the secondary antibody isAlexa Fluor 568 donkey anti - mouseIgG (invitrogen A10037) not the onei mentioned before. I used a red secondary
      I knowalexa 488 is green andalexa 568 is red andi used the right filter, let's just not keep on going on this point please. The filteri used was correct .
      Looking forward to you reply
      Best Wishes

      Read More
      Answer

      ers used and secondary antibody are correct. We always double check the protocol steps with our customer when some of them do not make sense. I apologize for any inconvenience.

      This antibody has been tested in ICC/IF with MCF7 cells and is fully guaranteed; the product should have worked however we are unsure about the problem. In order to help you further I am happy providing free of charge replacement product or a different antibody. Could you let me know how you would like to proceed.

      Looking forward to hearing from you soon.

      Read More

      Question

      a h Anti-alpha 1 Fetoprotein antibody (ab3980).
      Details as follow:
      LOT NUMBER: GR39372-3
      ORDER NUMBER: 304583
      DESCRIPTION OF THE PROBLEM
      No staining or very low stain
      SAMPLE
      Human hepatocytes derivated from iPSC
      Huh7 cell ines for positive control
      PRIMARY ANTIBODY Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)
      diluent: blocking buffer (0.5% BSA in PBS); dilution: tested at 1/50, 1/100 and 1/200; Incubation time: o/n at 4º; wash step: TBS 3x5 minuts each
      DETECTION METHOD
      Fluorescence microscope
      POSITIVE AND NEGATIVE CONTROLS USED
      positive: Huh7 Hepatocyte cell line (image attached)
      negative: Fibroblasts
      ANTIBODY STORAGE CONDITIONS
      shipped at 4º, aliquot and stored at -20º
      FIXATION OF SAMPLE
      PFA 4% for 15 min
      ANTIGEN RETRIEVAL
      Cell were immediately stained after fixation, no antigen retrieval was needed
      PERMEABILIZATION STEP
      0.1% Triton X-100 PBS
      BLOCKING CONDITIONS
      1h at RT in 0.5% BSA in PBS
      SECONDARY ANTIBODY
      tested with:
      Alexa 488 Donkey anti-mouse (invitrogen)
      diluent: blocking buffer (0.5% BSA in PBS)
      dilution: 1/1000
      Incubation time: 1 hour at RT
      wash step: PBS 3x5 minuts each
      HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3
      HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes
      DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes
      Pictures enclosed (dilution 1 in 50)

      Read More
      Answer

      ram very sorry to hear that you are experiencing problems with this antibody.

      The protocol looks fine to me however I have following further suggestions

      - We used methanol as fixative when we characterized this antibody. We recommend trying this.
      - It is mentioned in the protocol that Alexa 488 conjugated secondary was used, Alexa 488 is green in colour however the images shows red staining which could mean that wrong filters were used for observing the images. Could you try using correct filters.

      I hope these suggestions will be helpful. Should you have any problem using this antibody again, please do not hesitate to contact me.

      Read More

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