Anti-alpha 1 Fetoprotein 抗体 [AFP-01] (ab3980)
Key features and details
- Mouse monoclonal [AFP-01] to alpha 1 Fetoprotein
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-alpha 1 Fetoprotein antibody [AFP-01]
alpha 1 Fetoprotein 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [AFP-01] to alpha 1 Fetoprotein -
由来種
Mouse -
特異性
The antibody reacts with human alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus. -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Human alpha 1 Fetoprotein.
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ポジティブ・コントロール
- Hep G2 human hepatocellular carcinoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
Purified by precipitation and chromatography -
ポリ/モノ
モノクローナル -
クローン名
AFP-01 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
組織特異性
Plasma. Synthesized by the fetal liver and yolk sac. -
配列類似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
発生段階
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
翻訳後修飾
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
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別名
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
see all
画像
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ICC/IF image of ab3980 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3980, 5µg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
参考文献 (29)
ab3980 は 29 報の論文で使用されています。
- Lenz D et al. Generation of an iPSC line from a patient with infantile liver failure syndrome 2 due to mutations in NBAS: DHMCi004-A. Stem Cell Res 35:101398 (2019). PubMed: 30772683
- Wei J et al. Establishment and characterization of the GC-030-35 cell line derived from gastric hepatoid adenocarcinoma. Cancer Manag Res 11:1275-1287 (2019). PubMed: 30799954
- Jung-Klawitter S et al. Generation of 2 iPSC clones from a patient with DNAJC12 deficiency: DHMCi003-A and DHMCi003-B. Stem Cell Res 36:101402 (2019). PubMed: 30901742
- Lenz D et al. Generation of an induced pluripotent stem cell (iPSC) line, DHMCi005-A, from a patient with CALFAN syndrome due to mutations in SCYL1. Stem Cell Res 37:101428 (2019). PubMed: 30959346
- Tongkobpetch S et al. Generation of two human iPSC lines (MDCUi001-A and MDCUi001-B) from dermal fibroblasts of a Thai patient with X-linked osteogenesis imperfecta using integration-free Sendai virus. Stem Cell Res 39:101493 (2019). PubMed: 31326747