Anti-alpha 1 Antitrypsin 抗体, prediluted (ab922)
製品の概要
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製品名Anti-alpha 1 Antitrypsin antibody, prediluted
alpha 1 Antitrypsin 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to alpha 1 Antitrypsin, prediluted
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, IHC-P, IHC-Frmore details
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種交差性交差種: Rat, Dog, Human, Monkey
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免疫原
Alpha-1-antitrypsin isolated from human serum.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
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バッファーPreservative: 0.05% Sodium azide
Inert stabilizer -
Concentration information loading... -
精製度Protein A purified
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ポリ/モノポリクローナル
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ミエローマunknown
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-P | 1/1. | |
| IHC-Fr | 1/1. |
ターゲット情報
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機能Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). -
組織特異性Ubiquitous. Expressed in leukocytes and plasma.
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関連疾患Alpha-1-antitrypsin deficiency
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配列類似性Belongs to the serpin family.
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ドメインThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
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翻訳後修飾N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. -
細胞内局在Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
- Information by UniProt
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参照データベース
- Entrez Gene: 5265 Human
- Entrez Gene: 24648 Rat
- Omim: 107400 Human
- SwissProt: P01009 Human
- SwissProt: P17475 Rat
- Unigene: 525557 Human
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別名
- A1A antibody
- A1AT antibody
- A1AT_HUMAN antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody, prediluted (ab922)Human normal placenta. Staining is observed as extracellular and cytoplasmic. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody, prediluted (ab922)ICC/IF image of ab922 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab922, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
データシートおよび資料
参考文献
ab922 has not yet been referenced specifically in any publications.
