Anti-alpha 1 Antitrypsin 抗体, prediluted (ab922)

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製品の概要

  • 製品名

    Anti-alpha 1 Antitrypsin antibody, prediluted
    alpha 1 Antitrypsin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to alpha 1 Antitrypsin, prediluted

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IHC-P, IHC-Frmore details

  • 種交差性

    交差種: Rat, Dog, Human, Monkey

  • 免疫原

    Alpha-1-antitrypsin isolated from human serum.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/1.
IHC-Fr 1/1.

ターゲット情報

  • 機能

    Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

  • 組織特異性

    Ubiquitous. Expressed in leukocytes and plasma.

  • 関連疾患

    Alpha-1-antitrypsin deficiency

  • 配列類似性

    Belongs to the serpin family.

  • ドメイン

    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.

  • 翻訳後修飾

    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.

  • 細胞内局在

    Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Information by UniProt

  • 参照データベース

    • 別名

      • A1A antibody
      • A1AT antibody
      • A1AT_HUMAN antibody
      • AAT antibody
      • Alpha 1 antiproteinase antibody
      • Alpha 1 antitrypsin antibody
      • Alpha 1 antitrypsin null antibody
      • Alpha 1 protease inhibitor antibody
      • Alpha-1 protease inhibitor antibody
      • Alpha-1-antiproteinase antibody
      • alpha1 proteinase inhibitor antibody
      • Alpha1AT antibody
      • Dom1 antibody
      • PI antibody
      • PI1 antibody
      • PRO2275 antibody
      • Serine (or cysteine) proteinase inhibitor clade A member 1 antibody
      • Serine protease inhibitor 1-1 antibody
      • Serine protease inhibitor A1a antibody
      • Serpin A1 antibody
      • Serpin A1a antibody
      • Serpin peptidase inhibitor clade A member 1 antibody
      • Serpina1 antibody
      • Short peptide from AAT antibody
      • SPAAT antibody
      • Spi1-1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody, prediluted (ab922)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody, prediluted (ab922)
      Human normal placenta. Staining is observed as extracellular and cytoplasmic. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody, prediluted (ab922)
      Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody, prediluted (ab922)
      ICC/IF image of ab922 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab922, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    参考文献

    ab922 has not yet been referenced specifically in any publications.

    ab922 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1-3 of 3 Abreviews or Q&A

    Question

    I would like to know if your Anti-alpha 1 Antitrypsin antibody, prediluted (ab922) is specific as a hepatocyte marker. I would also want to know if there are publications you are aware of, citing the product.

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    Answer



    All the information and references available for our products are contained on its datasheet. These references are updated as soon as any new information is brought to our attention. Unfortunately we don’t have any references for this antibody for the time being.

    This antibody is a mature hepatocyte marker. Alternatives specific markers for these cells are albumin, Fetoprotein, Annexin I and Annexin II (useful markers for detecting not only actively proliferating hepatocytes but also hepatocytes in preproliferative and postproliferative stages).

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    Question

    I have a question concerning your product:alpha 1-Antitrypsin antibody, product code ab922. In the information about specifity you wrote that it stains only macrophages and it does't stain any other cell types. Is it true? Mayby you can tell me what are specific markers of differentiated hepatocyte? I am going to work with human hepatocyte cell line. Do you have any antibodies against such markers?

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    Answer

    This antibody reacts primarily with AAT because only one precipitating arch appears in immunoelectrophoresis. Contaminating antibodies were removed by solid phase absorption. I'm afraid that I am unable to advise which antibody would be suitable for use as a specific marker of differentiated hepatocytes.

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    Question

    1) With which other proteins have you tested the cross-reactivity of this antibody? May it be useful to target alpha1-antitrypsin from rats and hamsters? 2) Is there any chance that it may also work for ELISA?

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    Answer

    This antibody is sourced externally, therefore all the information we have for this product is stated on the datasheet. The datasheet says "The antibody is specific for human alpha-1-antitrypsin.......stains macrophages in human tissues and does not stain any other cell type.....cross reactivity against monkey, dog and rat. Not tested in other species". Since this antibody is polyclonal and raised to whole A1AT, it is likely that it will work in an ELISA, but we can't guarantee this until tested.

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